Construction of a novel phenol synthetic pathway in Escherichia coli through 4-hydroxybenzoate decarboxylation
文献类型：期刊论文
| 作者 | Miao, Liangtian1,2,3; Li, Qingyan2,3; Diao, Aipo1; Zhang, Xueli2,3; Ma, Yanhe2 |
| 刊名 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2015-06-01 |
| 卷号 | 99期号:12页码:5163-5173 |
| 关键词 | Phenol 4-hydroxybenzoate decarboxylase Two-phase extractive fermentation Escherichia coli |
| 英文摘要 | Phenol is a bulk chemical with lots of applications in the chemical industry. Fermentative production of phenol had been realized in both Pseudomonas putida and Escherichia coli by recruiting tyrosine phenol-lyase (TPL). The TPL pathway needs tyrosine as the direct precursor for phenol production. In this work, a novel phenol synthetic pathway was created in E. coli by recruiting 4-hydroxybenzoate decarboxylase, which can convert 4-hydroxybenzoate to phenol and carbon dioxide. Activating 3-deoxy-d-arabino-heptulosonate-7-phosphate (DAHP) synthase and chorismate pyruvate lyase (UbiC) through plasmid overexpression led to 7- and 69-fold increase of phenol production, respectively, demonstrating that these two enzymes were the rate-limiting steps for phenol production. Genetically stable strains were then obtained by gene integration and gene modulation directly in chromosome. Phenol titer increased 147-fold (from 1.7 to 250 mg/L) after modulating the DAHP synthase, UbiC, and 4-hydroxybenzoate decarboxylase genes in chromosome. Five solvents were tested for two-phase extractive fermentation to eliminate phenol toxicity to E. coli cells. Tributyrin and dibutyl phthalate were the best two solvents for improving phenol production, leading to 23 and 30 % increase of total phenol production, respectively. Two-phase fed-batch fermentation of the best strain Phe009 was performed in a 7 L fermentor, which produced 9.51 g/L phenol with a yield of 0.061 g/g glucose. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | PSEUDOMONAS-PUTIDA S12 ; L-PHENYLALANINE ; GLUCOSE ; GENES ; STRAINS ; ACID ; TRANSCRIPTION ; BIOSYNTHESIS ; PURIFICATION ; IMPROVEMENT |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000355208900018 |
| 源URL | [http://124.16.173.210/handle/834782/1480]  |
| 专题 | 天津工业生物技术研究所_微生物代谢工程 张学礼_期刊论文 |
| 作者单位 | 1.Tianjin Univ Sci & Technol, Tianjin 300457, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 3.Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Miao, Liangtian,Li, Qingyan,Diao, Aipo,et al. Construction of a novel phenol synthetic pathway in Escherichia coli through 4-hydroxybenzoate decarboxylation[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2015,99(12):5163-5173. |
| APA | Miao, Liangtian,Li, Qingyan,Diao, Aipo,Zhang, Xueli,&Ma, Yanhe.(2015).Construction of a novel phenol synthetic pathway in Escherichia coli through 4-hydroxybenzoate decarboxylation.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,99(12),5163-5173. |
| MLA | Miao, Liangtian,et al."Construction of a novel phenol synthetic pathway in Escherichia coli through 4-hydroxybenzoate decarboxylation".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 99.12(2015):5163-5173. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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