Transcriptome analysis of Chlamydomonas reinhardtii during the process of lipid accumulation
文献类型：期刊论文
| 作者 | Lv, Hexin1; Qu, Ge1; Qi, Xizhen1; Lu, Lina1; Tian, Chaoguang1; Ma, Yanhe1,2 |
| 刊名 | GENOMICS
 |
| 出版日期 | 2013-04-01 |
| 卷号 | 101期号:4页码:229-237 |
| 关键词 | Transcriptome RNA-seq Chlamydomonas Lipids Biodiesel |
| 英文摘要 | Algae are potential candidates for biodiesel production; thus, it is important to gain insight into the molecular mechanism of their lipid metabolism. Time-course transcriptome analyses were carried out during the lipid biosynthesis and accumulation processes of the model green alga Chiamydomonas reinhardtii using the Illumina RNA-seq platform. Transcriptome results indicated that over 2500 genes are upregulated or during lipid accumulation compared to log phase growth. As a proof of principle, two of the enzymes required for lipid metabolism that were significantly up-regulated during lipid accumulation, Lyso-Phosphatidic Acid Acyltransferase (LPAAT), diacylglycerol acyltransferase (DAGAT) were knocked down using artificial microRNAs. Neutral lipid production decreased in strains knocked down in expression of the Ipaat and dagat genes. In addition, forty-one transcription factors were up- or down-regulated during the lipid accumulation process. This transcriptome data will be useful for engineering economic algae species aimed at biodiesel production. (C) 2013 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| 关键词[WOS] | ARABIDOPSIS SEED MATURATION ; FATTY-ACID BIOSYNTHESIS ; REGULATORY NETWORKS ; BIOFUEL PRODUCTION ; OVER-EXPRESSION ; GENE-REGULATION ; GENOME REVEALS ; OIL CONTENT ; METABOLISM ; ACYLTRANSFERASE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000317252500004 |
| 源URL | [http://124.16.173.210/handle/834782/1240]  |
| 专题 | 天津工业生物技术研究所_微生物功能基因组学与生物技术实验室 田朝光_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 2.Chinese Acad Sci, Inst Microbiol, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lv, Hexin,Qu, Ge,Qi, Xizhen,et al. Transcriptome analysis of Chlamydomonas reinhardtii during the process of lipid accumulation[J]. GENOMICS,2013,101(4):229-237. |
| APA | Lv, Hexin,Qu, Ge,Qi, Xizhen,Lu, Lina,Tian, Chaoguang,&Ma, Yanhe.(2013).Transcriptome analysis of Chlamydomonas reinhardtii during the process of lipid accumulation.GENOMICS,101(4),229-237. |
| MLA | Lv, Hexin,et al."Transcriptome analysis of Chlamydomonas reinhardtii during the process of lipid accumulation".GENOMICS 101.4(2013):229-237. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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