Identification and characterization of a new acid-stable endoglucanase from a metagenomic library
文献类型：期刊论文
| 作者 | Xiang, La1; Li, Aiying3; Tian, Chaoguang2; Zhou, Yuling1; Zhang, Guimin1; Ma, Yanhe2 |
| 刊名 | PROTEIN EXPRESSION AND PURIFICATION
 |
| 出版日期 | 2014-10-01 |
| 卷号 | 102页码:20-26 |
| 关键词 | Endoglucanase Metagenomic library Enzymatic assay Prokaryotic expression |
| 英文摘要 | A new endoglucanase gene cel124 was cloned from a metagenomic library and expressed in Escherichia coli. Catalytic triad analysis showed that the catalytic triad sites were different from the known endoglucanases. Cel124, a 34 kDa protein, exhibited a specific activity (29.08 U mg(-1)) toward 1% of sodium carboxymethyl cellulose and was stable at 50 C for 30 mm. The optimal temperature and pH for its catalytic activity were 50 degrees C and pH 5.5 respectively. Cel124 could hydrolyze soluble cellulose, but not insoluble cellulose or other polysaccharides. The kinetic parameters (5.63 mg ml(-1) for K-m and 0.0397 mmol min(-1) mg(-1) for V-max) were measured. 3 M NaCl in the system could increase its activity by 2 fold. Site-directed mutation and circular dichroism spectra test suggested that the residue (Glu41) was essential for its activity, might be a potential active site. Based on our data, we proposed that Cell 24 might represent a new type of endoglucanase. (C) 2014 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 关键词[WOS] | CLOSTRIDIUM-THERMOCELLUM ; PURIFICATION ; CELLULASE ; GENE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000342481600004 |
| 源URL | [http://124.16.173.210/handle/834782/1348]  |
| 专题 | 天津工业生物技术研究所_微生物功能基因组学与生物技术实验室 田朝光_期刊论文 |
| 作者单位 | 1.Hubei Univ, Coll Life Sci, Collaborat Innovat Ctr Bioresource Green Transfor, Wuhan 430062, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 3.Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xiang, La,Li, Aiying,Tian, Chaoguang,et al. Identification and characterization of a new acid-stable endoglucanase from a metagenomic library[J]. PROTEIN EXPRESSION AND PURIFICATION,2014,102:20-26. |
| APA | Xiang, La,Li, Aiying,Tian, Chaoguang,Zhou, Yuling,Zhang, Guimin,&Ma, Yanhe.(2014).Identification and characterization of a new acid-stable endoglucanase from a metagenomic library.PROTEIN EXPRESSION AND PURIFICATION,102,20-26. |
| MLA | Xiang, La,et al."Identification and characterization of a new acid-stable endoglucanase from a metagenomic library".PROTEIN EXPRESSION AND PURIFICATION 102(2014):20-26. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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