Heterologous Expression and Characterization of a GH3 beta-Glucosidase from Thermophilic Fungi Myceliophthora thermophila in Pichia pastoris
文献类型：期刊论文
| 作者 | Zhao, Junqi; Guo, Chao; Tian, Chaoguang; Ma, Yanhe |
| 刊名 | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
 |
| 出版日期 | 2015-09-01 |
| 卷号 | 177期号:2页码:511-527 |
| 关键词 | Myceliophthora thermophila beta-Glucosidase Substrate specificity Transglycosylation activity Pichia pastoris |
| 英文摘要 | A novel beta-glucosidase of glycoside hydrolase (GH) family 3 from Myceliophthora thermophila (mtbgl3b) was successfully expressed in Pichia pastoris. The full-length gene consists of 2613 bp nucleotides encoding a protein of 870 amino acids. MtBgl3b showed maximum activity at pH 5.0 and remained more than 70 % relative activity at 3.5-6.0. The enzyme displayed the highest activity at 60 A degrees C and kept about 90 % relative activity for 50-65 A degrees C; besides, the enzyme showed psychrophilic trait and remains 51 % relative activity at 40 A degrees C. MtBgl3b exhibited good stability over a wide pH range of 3.0-10.0 and was thermostable at 60 and 65 A degrees C. The enzyme displayed highest activity towards p-nitrophenyl-beta-d-glucopyranoside (pNPG), followed by p-nitrophenyl-d-cellobioside (pNPC), cellotetraose, cellotriose, cellobiose, and gentiobiose. When using 10 % cellobiose (w/v) as the substrate, the enzyme showed transglycosylation activity to produce the cellotriose. The kinetic parametric of K (m) and V (max) were 2.78 mM and 927.9 mu M mg(-1) min(-1), respectively. Finally, the reaction mode of the enzyme and the substrates were analyzed by molecular docking approach. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 关键词[WOS] | FAMILY 3 ; PAECILOMYCES-THERMOPHILA ; FUNCTIONAL EXPRESSION ; THERMOSTABLE ENZYMES ; TRICHODERMA-REESEI ; ASPERGILLUS-NIGER ; CLONING ; GENE ; PURIFICATION ; HYDROLYSIS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000360076200016 |
| 源URL | [http://124.16.173.210/handle/834782/1519]  |
| 专题 | 天津工业生物技术研究所_微生物功能基因组学与生物技术实验室 田朝光_期刊论文 |
| 作者单位 | Chinese Acad Sci, Tianjin Airport Econ Area, Tianjin Inst Ind Biotechnol, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Junqi,Guo, Chao,Tian, Chaoguang,et al. Heterologous Expression and Characterization of a GH3 beta-Glucosidase from Thermophilic Fungi Myceliophthora thermophila in Pichia pastoris[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2015,177(2):511-527. |
| APA | Zhao, Junqi,Guo, Chao,Tian, Chaoguang,&Ma, Yanhe.(2015).Heterologous Expression and Characterization of a GH3 beta-Glucosidase from Thermophilic Fungi Myceliophthora thermophila in Pichia pastoris.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,177(2),511-527. |
| MLA | Zhao, Junqi,et al."Heterologous Expression and Characterization of a GH3 beta-Glucosidase from Thermophilic Fungi Myceliophthora thermophila in Pichia pastoris".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 177.2(2015):511-527. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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