Cloning, Characterization, and Production of a Novel Lysozyme by Different Expression Hosts
文献类型：期刊论文
| 作者 | Zhang, Haifeng1,2; Fu, Gang1,3; Zhang, Dawei1,2,3 |
| 刊名 | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2014-10-01 |
| 卷号 | 24期号:10页码:1405-1412 |
| 关键词 | Lysozyme Bacillus licheniformis Bacillus subtilis antimicrobial protein expression system |
| 英文摘要 | Lysozyme is a protein found in egg white, tears, saliva, and other secretions. As a marketable natural alternative to preservatives, lysozyme can act as a natural antibiotic. In this study, we have isolated Bacillus licheniformis TIB320 from soil, which contains a lysozyme gene with various features. We have cloned and expressed the lysozyme in E. coli. The antimicrobial activity of the lysozyme showed that it had a broad antimicrobial spectrum against several standard strains. The lysozyme could maintain efficient activities in a pH range between 3 and 9 and from 20 degrees C to 60 degrees C, respectively. The lysozyme was resistant to pepsin and trypsin to some extent at 40 degrees C. Production of the lysozyme was optimized by using various expression strategies in B. subtilis WB800. The lysozyme from B. licheniformis TIB320 will be promising as a food or feed additive. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Microbiology |
| 关键词[WOS] | EGG-WHITE LYSOZYME ; GRAM-NEGATIVE BACTERIA ; ESCHERICHIA-COLI ; BACILLUS-SUBTILIS ; DENTAL CEMENTS ; PROTEIN ; SYSTEM ; PURIFICATION ; GENE ; CELL |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000343784600013 |
| 源URL | [http://124.16.173.210/handle/834782/1363]  |
| 专题 | 天津工业生物技术研究所_微生物生理与蛋白质组学 张大伟_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 2.Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China 3.Natl Engn Lab Ind Enzymes, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Haifeng,Fu, Gang,Zhang, Dawei. Cloning, Characterization, and Production of a Novel Lysozyme by Different Expression Hosts[J]. JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY,2014,24(10):1405-1412. |
| APA | Zhang, Haifeng,Fu, Gang,&Zhang, Dawei.(2014).Cloning, Characterization, and Production of a Novel Lysozyme by Different Expression Hosts.JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY,24(10),1405-1412. |
| MLA | Zhang, Haifeng,et al."Cloning, Characterization, and Production of a Novel Lysozyme by Different Expression Hosts".JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY 24.10(2014):1405-1412. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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