A rapid enzymatic assay for high-throughput screening of adenosine-producing strains
文献类型：期刊论文
| 作者 | Dong, Huina1,2; Zu, Xin1,3; Zheng, Ping1,2; Zhang, Dawei1,2 |
| 刊名 | MICROBIAL BIOTECHNOLOGY
 |
| 出版日期 | 2015-03-01 |
| 卷号 | 8期号:2页码:230-238 |
| 英文摘要 | Adenosine is a major local regulator of tissue function and industrially useful as precursor for the production of medicinal nucleoside substances. High-throughput screening of adenosine overproducers is important for industrial microorganism breeding. An enzymatic assay of adenosine was developed by combined adenosine deaminase (ADA) with indophenol method. The ADA catalyzes the cleavage of adenosine to inosine and NH3, the latter can be accurately determined by indophenol method. The assay system was optimized to deliver a good performance and could tolerate the addition of inorganic salts and many nutrition components to the assay mixtures. Adenosine could be accurately determined by this assay using 96-well microplates. Spike and recovery tests showed that this assay can accurately and reproducibly determine increases in adenosine in fermentation broth without any pretreatment to remove proteins and potentially interfering low-molecular-weight molecules. This assay was also applied to high-throughput screening for high adenosine-producing strains. The high selectivity and accuracy of the ADA assay provides rapid and high-throughput analysis of adenosine in large numbers of samples. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Microbiology |
| 关键词[WOS] | NO-REFLOW PHENOMENON ; BACILLUS-SUBTILIS ; INTRACORONARY ADENOSINE ; REPERFUSION INJURY ; AMMONIA ; APTASENSOR ; SERUM ; CHROMATOGRAPHY ; ATTENUATION ; SYNTHETASE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000350345300004 |
| 源URL | [http://124.16.173.210/handle/834782/1437]  |
| 专题 | 天津工业生物技术研究所_微生物生理与蛋白质组学 张大伟_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 2.Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China 3.Dalian Polytech Univ, Sch Biol Engn, Light Ind Technol & Engn, Dalian 116034, Liaoning, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dong, Huina,Zu, Xin,Zheng, Ping,et al. A rapid enzymatic assay for high-throughput screening of adenosine-producing strains[J]. MICROBIAL BIOTECHNOLOGY,2015,8(2):230-238. |
| APA | Dong, Huina,Zu, Xin,Zheng, Ping,&Zhang, Dawei.(2015).A rapid enzymatic assay for high-throughput screening of adenosine-producing strains.MICROBIAL BIOTECHNOLOGY,8(2),230-238. |
| MLA | Dong, Huina,et al."A rapid enzymatic assay for high-throughput screening of adenosine-producing strains".MICROBIAL BIOTECHNOLOGY 8.2(2015):230-238. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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