Enhanced extracellular production of alpha-amylase in Bacillus subtilis by optimization of regulatory elements and over-expression of PrsA lipoprotein
文献类型：期刊论文
| 作者 | Chen, Jingqi1,2,3; Gai, Yuanming2,3; Fu, Gang2,3,4; Zhou, Wenjuan2,3; Zhang, Dawei2,3,4; Wen, Jianping1 |
| 刊名 | BIOTECHNOLOGY LETTERS
 |
| 出版日期 | 2015-04-01 |
| 卷号 | 37期号:4页码:899-906 |
| 关键词 | alpha-Amylase Bacillus subtilis Fed-batch fermentation Promoter PrsA Regulatory elements Signal peptide |
| 英文摘要 | alpha-Amylase was used as a heterologous model protein to investigate the effects of promoters, signal peptides and over-expression of an extra-cytoplasmic molecular chaperone, PrsA lipoprotein, on enhancing the secretion of alpha-amylase in Bacillus subtilis. Four promoters and six signal peptides were compared, successively, and the highest yield of alpha-amylase was achieved under the promotion mediated by P-AprE, a strong constitutive promoter, and secretion by SPnprE, a signal peptide from B. subtilis. Moreover, under conditions of overexpressed PrsA lipoprotein, the secretion production and activity of alpha-amylase increased to 2.5-fold. The performance of the recombinant B. subtilis 1A751PL31 was evaluated with a fed-batch fermentation in a 7.5 l fermentor. Optimization of regulatory elements and over-expression of PrsA lipoprotein had a significant effect on enhancing the production of alpha-amylase in B. subtilis. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | PROTEIN SECRETION ; ESCHERICHIA-COLI ; SIGNAL PEPTIDES ; LICHENIFORMIS ; CLONING ; GROWTH |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000351535300019 |
| 源URL | [http://124.16.173.210/handle/834782/1442]  |
| 专题 | 天津工业生物技术研究所_微生物生理与蛋白质组学 张大伟_期刊论文 |
| 作者单位 | 1.Tianjin Univ, Dept Biol Engn, Sch Chem Engn & Technol, Tianjin 300072, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 3.Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China 4.Natl Engn Lab Ind Enzymes, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Jingqi,Gai, Yuanming,Fu, Gang,et al. Enhanced extracellular production of alpha-amylase in Bacillus subtilis by optimization of regulatory elements and over-expression of PrsA lipoprotein[J]. BIOTECHNOLOGY LETTERS,2015,37(4):899-906. |
| APA | Chen, Jingqi,Gai, Yuanming,Fu, Gang,Zhou, Wenjuan,Zhang, Dawei,&Wen, Jianping.(2015).Enhanced extracellular production of alpha-amylase in Bacillus subtilis by optimization of regulatory elements and over-expression of PrsA lipoprotein.BIOTECHNOLOGY LETTERS,37(4),899-906. |
| MLA | Chen, Jingqi,et al."Enhanced extracellular production of alpha-amylase in Bacillus subtilis by optimization of regulatory elements and over-expression of PrsA lipoprotein".BIOTECHNOLOGY LETTERS 37.4(2015):899-906. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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