Combinatorial Sec pathway analysis for improved heterologous protein secretion in Bacillus subtilis: identification of bottlenecks by systematic gene overexpression
文献类型：期刊论文
| 作者 | Chen, Jingqi1,2,3; Fu, Gang2,4; Gai, Yuanming2,4; Zheng, Ping2,3; Zhang, Dawei2,3,4; Wen, Jianping1 |
| 刊名 | MICROBIAL CELL FACTORIES
 |
| 出版日期 | 2015-06-26 |
| 卷号 | 14 |
| 关键词 | Bacillus subtilis Bottleneck Heterologous protein Overexpression Sec pathway |
| 英文摘要 | Background: Secretory expression of valuable proteins by B. subtilis and its related species has attracted intensive work over the past three decades. Although very high yields can be achieved with homologous proteins, production of heterologous proteins by B. subtilis is unfortunately not the straight forward. The Sec pathway is the major route for protein secretion in B. subtilis. Therefore, the aim of this work was to identify the bottlenecks of the Sec pathway and improve the secretion of heterologous proteins by molecular genetic techniques. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | SMALL CYTOPLASMIC RNA ; HIGH-LEVEL SECRETION ; ALPHA-AMYLASE ; LACTOCOCCUS-LACTIS ; PRSA LIPOPROTEIN ; EXPRESSION ; TRANSFORMATION ; TRANSLOCATION ; DEGRADATION ; PARTICLE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000357026800002 |
| 源URL | [http://124.16.173.210/handle/834782/1499]  |
| 专题 | 天津工业生物技术研究所_微生物生理与蛋白质组学 张大伟_期刊论文 |
| 作者单位 | 1.Tianjin Univ, Sch Chem Engn & Technol, Dept Biol Engn, Tianjin 300072, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 3.Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China 4.Natl Engn Lab Ind Enzymes, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Jingqi,Fu, Gang,Gai, Yuanming,et al. Combinatorial Sec pathway analysis for improved heterologous protein secretion in Bacillus subtilis: identification of bottlenecks by systematic gene overexpression[J]. MICROBIAL CELL FACTORIES,2015,14. |
| APA | Chen, Jingqi,Fu, Gang,Gai, Yuanming,Zheng, Ping,Zhang, Dawei,&Wen, Jianping.(2015).Combinatorial Sec pathway analysis for improved heterologous protein secretion in Bacillus subtilis: identification of bottlenecks by systematic gene overexpression.MICROBIAL CELL FACTORIES,14. |
| MLA | Chen, Jingqi,et al."Combinatorial Sec pathway analysis for improved heterologous protein secretion in Bacillus subtilis: identification of bottlenecks by systematic gene overexpression".MICROBIAL CELL FACTORIES 14(2015). |
入库方式： OAI收割
来源：天津工业生物技术研究所
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