Engineering of recombinant Escherichia coli cells co-expressing poly-gamma-glutamic acid (gamma-PGA) synthetase and glutamate racemase for differential yielding of gamma-PGA
文献类型：期刊论文
| 作者 | Cao, Mingfeng1,2; Geng, Weitao1; Zhang, Wei1; Sun, Jibin2; Wang, Shufang3; Feng, Jun1; Zheng, Ping2; Jiang, Anna2; Song, Cunjiang1 |
| 刊名 | MICROBIAL BIOTECHNOLOGY
 |
| 出版日期 | 2013-11-01 |
| 卷号 | 6期号:6页码:675-684 |
| 英文摘要 | Poly--glutamic acid (-PGA) is a promising environmental-friendly material with outstanding water solubility, biocompatibility and degradability. However, it is tough to determine the relationship between functional synthetic enzyme and the strains' yield or substrate dependency. We cloned -PGA synthetase genes pgsBCA and glutamate racemase gene racE from both L-glutamate-dependent -PGA-producing Bacillus licheniformisNK-03 and L-glutamate-independent B.amyloliquefaciensLL3 strains. The deduced RacE and PgsA from the two strains shared the identity of 84.5% and 78.53%, while PgsB and PgsC possessed greater similarity with 93.13% and 93.96%. The induced co-expression of pgsBCA and racE showed that the engineered Escherichia coli strains had the capacity of synthesizing -PGA, and LL3 derived PgsBCA had higher catalytic activity and enhanced productivity than NK-03 in Luria-Bertani medium containing glucose or L-glutamate. However, the differential effect was weakened when providing sufficient immediateness L-glutamate substrate, that is, the supply of substrate could be served as the ascendance upon -PGA production. Furthermore, RacE integration could enhance -PGA yield through improving the preferred d-glutamate content. This is the first report about co-expression of pgsBCA and racE from the two Bacillus strains, which will be of great value for the determination of the biosynthetic mechanism of -PGA. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Microbiology |
| 关键词[WOS] | BACILLUS-SUBTILIS ; POLY(GAMMA-GLUTAMIC ACID) ; INDEPENDENT PRODUCTION ; POLYGLUTAMIC ACID ; LICHENIFORMIS ; BIOSYNTHESIS ; COMPLEX ; GENE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000325546400006 |
| 源URL | [http://124.16.173.210/handle/834782/1262]  |
| 专题 | 天津工业生物技术研究所_系统与合成生物技术实验室 孙际宾_期刊论文 |
| 作者单位 | 1.Nankai Univ, Minist Educ, Key Lab Mol Microbiol & Technol, Tianjin 300071, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 3.Nankai Univ, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cao, Mingfeng,Geng, Weitao,Zhang, Wei,et al. Engineering of recombinant Escherichia coli cells co-expressing poly-gamma-glutamic acid (gamma-PGA) synthetase and glutamate racemase for differential yielding of gamma-PGA[J]. MICROBIAL BIOTECHNOLOGY,2013,6(6):675-684. |
| APA | Cao, Mingfeng.,Geng, Weitao.,Zhang, Wei.,Sun, Jibin.,Wang, Shufang.,...&Song, Cunjiang.(2013).Engineering of recombinant Escherichia coli cells co-expressing poly-gamma-glutamic acid (gamma-PGA) synthetase and glutamate racemase for differential yielding of gamma-PGA.MICROBIAL BIOTECHNOLOGY,6(6),675-684. |
| MLA | Cao, Mingfeng,et al."Engineering of recombinant Escherichia coli cells co-expressing poly-gamma-glutamic acid (gamma-PGA) synthetase and glutamate racemase for differential yielding of gamma-PGA".MICROBIAL BIOTECHNOLOGY 6.6(2013):675-684. |
入库方式： OAI收割
来源：天津工业生物技术研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。