Analysis of Enhanced Current-Generating Mechanism of Geobacter sulfurreducens Strain via Model-Driven Metabolism Simulation
文献类型：期刊论文
| 作者 | Meng, Jing1; Xu, Zixiang2; Guo, Jing1; Yue, Yunxia1; Sun, Xiao1 |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2013-09-13 |
| 卷号 | 8期号:9 |
| 英文摘要 | Microbial fuel cells (MFCs) are a class of ideal technologies that function via anaerobic respiration of electricigens, which bring current generation and environmental restoration together. An in-depth understanding of microbial metabolism is of great importance in engineering microbes to further improve their respiration. We employed flux balance analysis and selected Fe(iii) as a substitute for the electrode to simulate current-generating metabolism of Geobacter sulfurreducens PCA with a fixed acetate uptake rate. Simulation results indicated the fluxes of reactions directing acetate towards dissimilation to generate electrons increased under the suboptimal growth condition, resulting in an increase in the respiration rate and a decrease in the growth rate. The results revealed the competitive relationship between oxidative respiration and cell growth during the metabolism of microbe current generation. The results helped us quantitatively understand why microbes growing slowly have the potential to make good use of fuel in MFCs. At the same time, slow growth does not necessarily result in speedy respiration. Alternative respirations may exist under the same growth state due to redundant pathways in the metabolic network. The big difference between the maximum and minimum respiration mainly results from the total formate secretion. With iterative flux variability analysis, a relatively ideal model of variant of G. sulfurreducens PCA was reconstructed by deleting several enzymes in the wild model, which could reach simultaneous suboptimal growth and maximum respiration. Under this ideal condition, flux towards extracellular electron transfer rather than for biosynthesis is beneficial for the conversion of organic matter to electricity without large accumulations of biomass and electricigens may maximize utilization of limited fuel. Our simulations will provide an insight into the enhanced current-generating mechanism and identify theoretical range of respiration rates for guiding strain improvement in MFCs. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | MICROBIAL FUEL-CELLS ; FLUX BALANCE ANALYSIS ; C-TYPE CYTOCHROME ; ELECTRON-TRANSFER ; EVOLUTION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000324408400040 |
| 源URL | [http://124.16.173.210/handle/834782/1269]  |
| 专题 | 天津工业生物技术研究所_系统与合成生物技术实验室 孙际宾_期刊论文 |
| 作者单位 | 1.Southeast Univ, State Key Lab Bioelect, Nanjing, Jiangsu, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Key Lab Syst Microbial Biotechnol, Tianjin, Peoples R China |
| 推荐引用方式 GB/T 7714 | Meng, Jing,Xu, Zixiang,Guo, Jing,et al. Analysis of Enhanced Current-Generating Mechanism of Geobacter sulfurreducens Strain via Model-Driven Metabolism Simulation[J]. PLOS ONE,2013,8(9). |
| APA | Meng, Jing,Xu, Zixiang,Guo, Jing,Yue, Yunxia,&Sun, Xiao.(2013).Analysis of Enhanced Current-Generating Mechanism of Geobacter sulfurreducens Strain via Model-Driven Metabolism Simulation.PLOS ONE,8(9). |
| MLA | Meng, Jing,et al."Analysis of Enhanced Current-Generating Mechanism of Geobacter sulfurreducens Strain via Model-Driven Metabolism Simulation".PLOS ONE 8.9(2013). |
入库方式： OAI收割
来源：天津工业生物技术研究所
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