Cloning of two 5-aminolevulinic acid synthase isozymes HemA and HemO from Rhodopseudomonas palustris with favorable characteristics for 5-aminolevulinic acid production
文献类型：期刊论文
| 作者 | Zhang, Lilu1,2,3; Chen, Jiuzhou2,3; Chen, Ning1; Sun, Jibin2,3; Zheng, Ping2,3; Ma, Yanhe3 |
| 刊名 | BIOTECHNOLOGY LETTERS
 |
| 出版日期 | 2013-05-01 |
| 卷号 | 35期号:5页码:763-768 |
| 关键词 | 5-Aminolevulinic acid production 5-Aminolevulinic acid synthase Hemin Rhodopseudomonas palustris |
| 英文摘要 | 5-Aminolevulinic acid (ALA) synthase (ALAS) HemA from non-sulfur photosynthetic bacteria has been used for the ALA bioproduction, whereas the isoenzyme HemT/HemO is less studied and not used for ALA production. Two ALAS-encoding genes, hemA and hemO from Rhodopseudomonas palustris were cloned, purified and characterized. The ALASs had very high specific activity, 3.6 and 2.7 U/mg, respectively, and strong affinity for one of its substrates, succinyl-CoA, K (m) with values of 11 and 4.4 mu M, respectively. HemO retained up to 60 % maximum activity within a broad range of concentrations of hemin, while HemA kept only 20 % at 10 mu M hemin. Escherichia coli overexpressing HemA or HemO produced 5.7 and 6.3 g ALA/l, respectively, in a 5 l bioreactor. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | RECOMBINANT ESCHERICHIA-COLI ; DELTA-AMINOLEVULINIC-ACID ; LIQUID-CHROMATOGRAPHY ; OPTIMIZATION ; KUGB306 ; GENE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000318510500015 |
| 源URL | [http://124.16.173.210/handle/834782/1295]  |
| 专题 | 天津工业生物技术研究所_系统与合成生物技术实验室 孙际宾_期刊论文 |
| 作者单位 | 1.Tianjin Univ Sci & Biotechnol, Coll Biotechnol, Tianjin 300457, Peoples R China 2.Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China 3.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Natl Engn Lab Ind Enzymes, Tianjin Airport Econ Area, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Lilu,Chen, Jiuzhou,Chen, Ning,et al. Cloning of two 5-aminolevulinic acid synthase isozymes HemA and HemO from Rhodopseudomonas palustris with favorable characteristics for 5-aminolevulinic acid production[J]. BIOTECHNOLOGY LETTERS,2013,35(5):763-768. |
| APA | Zhang, Lilu,Chen, Jiuzhou,Chen, Ning,Sun, Jibin,Zheng, Ping,&Ma, Yanhe.(2013).Cloning of two 5-aminolevulinic acid synthase isozymes HemA and HemO from Rhodopseudomonas palustris with favorable characteristics for 5-aminolevulinic acid production.BIOTECHNOLOGY LETTERS,35(5),763-768. |
| MLA | Zhang, Lilu,et al."Cloning of two 5-aminolevulinic acid synthase isozymes HemA and HemO from Rhodopseudomonas palustris with favorable characteristics for 5-aminolevulinic acid production".BIOTECHNOLOGY LETTERS 35.5(2013):763-768. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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