Why mosaic? Gene expression profiling of African cassava mosaic virus-infected cassava reveals the effect of chlorophyll degradation on symptom development
文献类型：期刊论文
| 作者 | Liu, Jiao1,2,3; Yang, Jun1; Bi, Huiping4; Zhang, Peng1,2,3 |
| 刊名 | JOURNAL OF INTEGRATIVE PLANT BIOLOGY
 |
| 出版日期 | 2014-02-01 |
| 卷号 | 56期号:2页码:122-132 |
| 关键词 | African cassava mosaic virus cassava chlorophyll degradation digital gene expression mosaic symptom |
| 英文摘要 | Cassava mosaic disease, caused by cassava begomoviruses, is the most serious disease for cassava in Africa. However, the pathogenesis of this disease is poorly understood. We employed high throughput digital gene expression profiling based on the Illumina Solexa sequencing technology to investigate the global transcriptional response of cassava to African cassava mosaic virus infection. We found that 3,210 genes were differentially expressed in virus-infected cassava leaves. Gene ontology term and Kyoto Encyclopedia of Genes and Genomes pathway analysis indicated that genes implicated in photosynthesis were most affected, consistent with the chlorotic symptoms observed in infected leaves. The upregulation of chlorophyll degradation genes, including the genes encoding chlorophyllase, pheophytinase, and pheophorbide a oxygenase, and downregulation of genes encoding the major apoproteins in light-harvesting complex II were confirmed by qRT-PCR. These findings, together with the reduction of chlorophyll b content and fewer grana stacks in the infected leaf cells, reveal that the degradation of chlorophyll plays an important role in African cassava mosaic virus symptom development. This study will provide a road map for future investigations into viral pathogenesis. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Plant Sciences |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Plant Sciences |
| 关键词[WOS] | OXYGEN-EVOLVING COMPLEX ; LIGHT-HARVESTING COMPLEXES ; EUPATORIUM-MAKINOI ; PROTEIN COMPLEXES ; PHOTOSYSTEM-II ; PHOTOSYNTHETIC PROPERTIES ; NICOTIANA-BENTHAMIANA ; ARABIDOPSIS-THALIANA ; GROWTH IRRADIANCE ; LEAF SENESCENCE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000341712700003 |
| 源URL | [http://124.16.173.210/handle/834782/1345]  |
| 专题 | 天津工业生物技术研究所_系统与合成生物技术实验室 孙际宾_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Chenshan Bot Garden, Shanghai Chenshan Plant Sci Res Ctr, Shanghai 201602, Peoples R China 2.Chinese Acad Sci, Natl Key Lab Plant Mol Genet, Shanghai 200032, Peoples R China 3.Chinese Acad Sci, Natl Ctr Plant Gene Res Shanghai, Inst Plant Physiol & Ecol, Shanghai Inst Biol Sci, Shanghai 200032, Peoples R China 4.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Jiao,Yang, Jun,Bi, Huiping,et al. Why mosaic? Gene expression profiling of African cassava mosaic virus-infected cassava reveals the effect of chlorophyll degradation on symptom development[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2014,56(2):122-132. |
| APA | Liu, Jiao,Yang, Jun,Bi, Huiping,&Zhang, Peng.(2014).Why mosaic? Gene expression profiling of African cassava mosaic virus-infected cassava reveals the effect of chlorophyll degradation on symptom development.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,56(2),122-132. |
| MLA | Liu, Jiao,et al."Why mosaic? Gene expression profiling of African cassava mosaic virus-infected cassava reveals the effect of chlorophyll degradation on symptom development".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 56.2(2014):122-132. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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