中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Determination of key enzymes for threonine synthesis through in vitro metabolic pathway analysis

文献类型:期刊论文

作者Zhang, Yanfei1; Meng, Qinglong1,2; Ma, Hongwu1; Liu, Yongfei1; Cao, Guoqiang1; Zhang, Xiaoran1; Zheng, Ping1; Sun, Jibin1; Zhang, Dawei1; Jiang, Wenxia1
刊名MICROBIAL CELL FACTORIES
出版日期2015-06-13
卷号14
关键词Key enzyme Metabolic control analysis Threonine synthesis Proteomics Flux control coefficient
英文摘要Background: The overexpression of key enzymes in a metabolic pathway is a frequently used genetic engineering strategy for strain improvement. Metabolic control analysis has been proposed to quantitatively determine key enzymes. However, the lack of quality data often makes it difficult to correctly identify key enzymes through control analysis. Here, we proposed a method combining in vitro metabolic pathway analysis and proteomics measurement to find the key enzymes in threonine synthesis pathway.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biotechnology & Applied Microbiology
研究领域[WOS]Biotechnology & Applied Microbiology
关键词[WOS]ESCHERICHIA-COLI ; SYSTEMS ; CELL ; RECONSTITUTION ; PROTEOMICS ; FLUX
收录类别SCI
语种英语
WOS记录号WOS:000356235300001
源URL[http://124.16.173.210/handle/834782/1493]  
专题天津工业生物技术研究所_系统与合成生物技术实验室 孙际宾_期刊论文
作者单位1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China
2.Tianjin Univ, Sch Chem Engn & Technol, Dept Biochem Engn, Key Lab Syst Bioengn,Minist Educ, Tianjin 300072, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Yanfei,Meng, Qinglong,Ma, Hongwu,et al. Determination of key enzymes for threonine synthesis through in vitro metabolic pathway analysis[J]. MICROBIAL CELL FACTORIES,2015,14.
APA Zhang, Yanfei.,Meng, Qinglong.,Ma, Hongwu.,Liu, Yongfei.,Cao, Guoqiang.,...&Ma, Yanhe.(2015).Determination of key enzymes for threonine synthesis through in vitro metabolic pathway analysis.MICROBIAL CELL FACTORIES,14.
MLA Zhang, Yanfei,et al."Determination of key enzymes for threonine synthesis through in vitro metabolic pathway analysis".MICROBIAL CELL FACTORIES 14(2015).

入库方式: OAI收割

来源:天津工业生物技术研究所

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