Developing a high-throughput screening method for threonine overproduction based on an artificial promoter
文献类型：期刊论文
| 作者 | Liu, Ya'nan1,2,3; Li, Qinggang2,3; Zheng, Ping2,3; Zhang, Zhidan3; Liu, Yongfei2,3; Sun, Cunmin2,3; Cao, Guoqiang2,3; Zhou, Wenjuan2,3; Wang, Xiaowei4; Zhang, Dawei2,3 |
| 刊名 | MICROBIAL CELL FACTORIES
 |
| 出版日期 | 2015-08-22 |
| 卷号 | 14 |
| 关键词 | High-throughput screening Threonine biosensor Threonine production Escherichia coli |
| 英文摘要 | Background: L-Threonine is an important amino acid for animal feed. Though the industrial fermentation technology of threonine achieved a very high level, there is still significant room to further improve the industrial strains. The biosensor-based high-throughput screening (HTS) technology has demonstrated its powerful applications. Unfortunately, for most of valuable fine chemicals such as threonine, a HTS system has not been established mainly due to the absence of a suitable biosensor. In this study, we developed a HTS method to gain high-yielding threonine-producing strains. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | SINGLE-CELL LEVEL ; ESCHERICHIA-COLI ; SALMONELLA-TYPHIMURIUM ; METABOLITE CONCENTRATIONS ; CYSB PROTEIN ; AMINO-ACIDS ; EVOLUTION ; INTEGRATION ; METHIONINE ; BACTERIAL |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000360282100001 |
| 源URL | [http://124.16.173.210/handle/834782/1517]  |
| 专题 | 天津工业生物技术研究所_系统与合成生物技术实验室 孙际宾_期刊论文 |
| 作者单位 | 1.Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin 300222, Peoples R China 2.Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China 3.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 4.Nanjing Tech Univ, Sch Pharmaceut Sci, Nanjing 211800, Jiangsu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Ya'nan,Li, Qinggang,Zheng, Ping,et al. Developing a high-throughput screening method for threonine overproduction based on an artificial promoter[J]. MICROBIAL CELL FACTORIES,2015,14. |
| APA | Liu, Ya'nan.,Li, Qinggang.,Zheng, Ping.,Zhang, Zhidan.,Liu, Yongfei.,...&Ma, Yanhe.(2015).Developing a high-throughput screening method for threonine overproduction based on an artificial promoter.MICROBIAL CELL FACTORIES,14. |
| MLA | Liu, Ya'nan,et al."Developing a high-throughput screening method for threonine overproduction based on an artificial promoter".MICROBIAL CELL FACTORIES 14(2015). |
入库方式： OAI收割
来源：天津工业生物技术研究所
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