DNA orientation-specific adhesion and patterning of living mammalian cells on self-assembled DNA monolayers
文献类型：期刊论文
| 作者 | Wang, SP; Cai, XQ; Wang, LH; Li, J; Li, Q; Zuo, XL; Shi, JY; Huang, Q; Fan, CH |
| 刊名 | CHEMICAL SCIENCE
 |
| 出版日期 | 2016 |
| 卷号 | 7期号:4页码:2722—2727 |
| 关键词 | SINGLE-STRANDED-DNA CANCER-CELLS SURFACE GOLD FORCES MICROENVIRONMENT DIFFERENTIATION GRADIENTS MOLECULES INTEGRIN |
| ISSN号 | 2041-6520 |
| 通讯作者 | Zuo, XL (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai 201800, Peoples R China. ; Zuo, XL (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Bioimaging Ctr, Shanghai Synchrotron Radiat Facil,CAS Key Lab Int, Shanghai 201800, Peoples R China. |
| 英文摘要 | To better understand cell behaviors on substrates, the precise control of density and orientation of cell-specific ligands remains a great challenge. In this study, we established an easy-to-use approach to manipulate the adhesion and patterning of mammalian cells on gold substrates. We prepared DNA self-assembled monolayers (DNA-SAMs) on gold substrates and found that the sequence-specific orientation of DNA-SAMs played an important role in modulating cell adhesion. We also found that the DNA-SAMs on gold substrates could be used as a potentially universal cell culture substrate, which showed properties similar to cationic polymers (e.g. poly-L lysine, PLL) substrates. Furthermore, we could manipulate cell adhesion by tuning the length of poly adenine (polyA) in the DNA sequence. We also prepared a DNA aptamer-based SAM to regulate cell adhesion by exploiting stimuli-responsive conformational change of the aptamer. By using the well-established DNA spotting technology, we patterned cells on DNA-SAMs to form a spot matrix and four English letters "CELL". Our findings suggest that DNA-SAMs on gold substrates are potentially useful for making smart surfaces for cell studies, thus introducing a new platform for cell/tissue engineering research. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000372614800034 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/25690]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Wang, SP,Cai, XQ,Wang, LH,et al. DNA orientation-specific adhesion and patterning of living mammalian cells on self-assembled DNA monolayers[J]. CHEMICAL SCIENCE,2016,7(4):2722—2727. |
| APA | Wang, SP.,Cai, XQ.,Wang, LH.,Li, J.,Li, Q.,...&Fan, CH.(2016).DNA orientation-specific adhesion and patterning of living mammalian cells on self-assembled DNA monolayers.CHEMICAL SCIENCE,7(4),2722—2727. |
| MLA | Wang, SP,et al."DNA orientation-specific adhesion and patterning of living mammalian cells on self-assembled DNA monolayers".CHEMICAL SCIENCE 7.4(2016):2722—2727. |
入库方式： OAI收割
来源：上海应用物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。