Equally sloped X-ray microtomography of living insects with low radiation dose and improved resolution capability
文献类型：期刊论文
| 作者 | Yao, SK; Fan, JD; Zong, YB; He, Y; Zhou, GZ; Sun, ZB; Zhang, JH; Huang, QJ; Xiao, TQ; Jiang, HD |
| 刊名 | APPLIED PHYSICS LETTERS
 |
| 出版日期 | 2016 |
| 卷号 | 108期号:12页码:— |
| 关键词 | PHASE-CONTRAST TOMOGRAPHY MICROSCOPY RECONSTRUCTION RETRIEVAL |
| ISSN号 | 0003-6951 |
| 通讯作者 | Jiang, HD (reprint author), Shandong Univ, State Key Lab Crystal Mat, Jinan 250100, Peoples R China. |
| 英文摘要 | Three-dimensional X-ray imaging of living specimens is challenging due to the limited resolution of conventional absorption contrast X-ray imaging and potential irradiation damage of biological specimens. In this letter, we present microtomography of a living specimen combining phase-contrast imaging and a Fourier-based iterative algorithm termed equally sloped tomography. Non-destructive 3D imaging of an anesthetized living yellow mealworm Tenebrio molitor was demonstrated with a relatively low dose using synchrotron generated X-rays. Based on the high-quality 3D images, branching tracheoles and different tissues of the insect in a natural state were identified and analyzed, demonstrating a significant advantage of the technique over conventional X-ray radiography or histotomy. Additionally, the insect survived without problem after a 1.92-s X-ray exposure and subsequent absorbed radiation dose of similar to 1.2 Gy. No notable physiological effects were observed after reviving the insect from anesthesia. The improved static tomographic method demonstrated in this letter shows advantage in the non-destructive structural investigation of living insects in three dimensions due to the low radiation dose and high resolution capability, and offers many potential applications in biological science. (C) 2016 AIP Publishing LLC. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000373348000045 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/25718]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Yao, SK,Fan, JD,Zong, YB,et al. Equally sloped X-ray microtomography of living insects with low radiation dose and improved resolution capability[J]. APPLIED PHYSICS LETTERS,2016,108(12):—. |
| APA | Yao, SK.,Fan, JD.,Zong, YB.,He, Y.,Zhou, GZ.,...&Jiang, HD.(2016).Equally sloped X-ray microtomography of living insects with low radiation dose and improved resolution capability.APPLIED PHYSICS LETTERS,108(12),—. |
| MLA | Yao, SK,et al."Equally sloped X-ray microtomography of living insects with low radiation dose and improved resolution capability".APPLIED PHYSICS LETTERS 108.12(2016):—. |
入库方式： OAI收割
来源：上海应用物理研究所
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