High-Sensitivity and High-Efficiency Detection of DNA Hydroxymethylation in Genomic DNA by Multiplexing Electrochemical Biosensing
文献类型：期刊论文
| 作者 | Chen, SX; Dou, YZ; Zhao, ZH; Li, FW; Su, J; Fan, CH; Song, SP |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2016 |
| 卷号 | 88期号:7页码:3476—3480 |
| 关键词 | PRINTED AMPEROMETRIC BIOSENSOR MAMMALIAN DNA TET PROTEINS 5-HYDROXYMETHYLCYTOSINE METHYLATION 5-METHYLCYTOSINE BRAIN 5-CARBOXYLCYTOSINE QUANTIFICATION CANCER |
| ISSN号 | 0003-2700 |
| 通讯作者 | Fan, CH ; Song, SP (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai 201800, Peoples R China. ; Fan, CH ; Song, SP (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Bioimaging Ctr, Shanghai Synchrotron Radiat Facil,CAS Key Lab Int, Shanghai 201800, Peoples R China. |
| 英文摘要 | DNA hydroxymethylation (5-hmC) is a kind of new epigenetic modification, which plays key roles in DNA demethylation, genomic reprogramming, and the gene expression in mammals. For further exploring the functions of 5-hmC, it is necessary to develop sensitive and selective methods for detecting 5-hmC. Herein, we developed a novel multiplexing electrochemical (MEC) biosensor for 5-hmC detection based on the glycosylation modification of 5-hmC and enzymatic signal amplification. The 5-hmC was first glycosylated by T4 beta-glucosyltransferase and then oxidated by sodium periodate. The resulting glucosyl-modified 5-hmC (5-ghmC) was incubated with ARP-biotin and was bound to avidin-HRP. The 5-hmC can be detected at the subnanogram level. Finally, we performed 5-hmC detection for mouse tissue samples and cancer cell lines. The limit of detection of the MEC biosensor is 20 times lower than that of commercial kits based on optical meaurement. Also, the biosensor presented high detection specificity because the chemical reaction for 5-hmC modification can not happen at any other unhydroxymethylated nucleic acid bases. Importantly, benefited by its multiplexing capacity, the developed MEC biosensor showed excellent high efficiency, which was time-saving and cost less. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000373656300011 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/25746]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Chen, SX,Dou, YZ,Zhao, ZH,et al. High-Sensitivity and High-Efficiency Detection of DNA Hydroxymethylation in Genomic DNA by Multiplexing Electrochemical Biosensing[J]. ANALYTICAL CHEMISTRY,2016,88(7):3476—3480. |
| APA | Chen, SX.,Dou, YZ.,Zhao, ZH.,Li, FW.,Su, J.,...&Song, SP.(2016).High-Sensitivity and High-Efficiency Detection of DNA Hydroxymethylation in Genomic DNA by Multiplexing Electrochemical Biosensing.ANALYTICAL CHEMISTRY,88(7),3476—3480. |
| MLA | Chen, SX,et al."High-Sensitivity and High-Efficiency Detection of DNA Hydroxymethylation in Genomic DNA by Multiplexing Electrochemical Biosensing".ANALYTICAL CHEMISTRY 88.7(2016):3476—3480. |
入库方式： OAI收割
来源：上海应用物理研究所
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