Conditional entropy in variation-adjusted windows detects selection signatures associated with expression quantitative trait loci (eQTLs)
文献类型：期刊论文
| 作者 | Handelman, SK; Seweryn, M; Smith, RM; Hartmann, K; Wang, DX; Pietrzak, M; Johnson, AD; Kloczkowski, A; Sadee, W |
| 刊名 | BMC GENOMICS
 |
| 出版日期 | 2015 |
| 卷号 | 16期号:0页码:S8 |
| 通讯作者 | Handelman, SK (reprint author), Ohio State Univ, Coll Med, Ctr Pharmacogen, Graves Hall,330 W 10th Ave, Columbus, OH 43210 USA. |
| 英文摘要 | Background: Over the past 50,000 years, shifts in human-environmental or human-human interactions shaped genetic differences within and among human populations, including variants under positive selection. Shaped by environmental factors, such variants influence the genetics of modern health, disease, and treatment outcome. Because evolutionary processes tend to act on gene regulation, we test whether regulatory variants are under positive selection. We introduce a new approach to enhance detection of genetic markers undergoing positive selection, using conditional entropy to capture recent local selection signals. Results We use conditional logistic regression to compare our Adjusted Haplotype Conditional Entropy (H vertical bar H) measure of positive selection to existing positive selection measures. H vertical bar H and existing measures were applied to published regulatory variants acting in cis (cis-eQTLs), with conditional logistic regression testing whether regulatory variants undergo stronger positive selection than the surrounding gene. These cis-eQTLs were drawn from six independent studies of genotype and RNA expression. The conditional logistic regression shows that, overall, H vertical bar H is substantially more powerful than existing positive-selection methods in identifying cis-eQTLs against other Single Nucleotide Polymorphisms (SNPs) in the same genes. When broken down by Gene Ontology, H vertical bar H predictions are particularly strong in some biological process categories, where regulatory variants are under strong positive selection compared to the bulk of the gene, distinct from those GO categories under overall positive selection.. However, cis-eQTLs in a second group of genes lack positive selection signatures detectable by H vertical bar H, consistent with ancient short haplotypes compared to the surrounding gene (for example, in innate immunity GO: 0042742); under such other modes of selection, H vertical bar H would not be expected to be a strong predictor.. These conditional logistic regression models are adjusted for Minor allele frequency(MAF); otherwise, ascertainment bias is a huge factor in all eQTL data sets. Relationships between Gene Ontology categories, positive selection and eQTL specificity were replicated with H vertical bar H in a single larger data set. Our measure, Adjusted Haplotype Conditional Entropy (H vertical bar H), was essential in generating all of the results above because it: 1) is a stronger overall predictor for eQTLs than comparable existing approaches, and 2) shows low sequential auto-correlation, overcoming problems with convergence of these conditional regression statistical models. Conclusions: Our new method, H vertical bar H, provides a consistently more robust signal associated with cis-eQTLs compared to existing methods. We interpret this to indicate that some cis-eQTLs are under positive selection compared to their surrounding genes. Conditional entropy indicative of a selective sweep is an especially strong predictor of eQTLs for genes in several biological processes of medical interest. Where conditional entropy is a weak or negative predictor of eQTLs, such as innate immune genes, this would be consistent with balancing selection acting on such eQTLs over long time periods. Different measures of selection may be needed for variant prioritization under other modes of evolutionary selection. |
| 学科主题 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| 关键词[WOS] | TRANSCRIPTIONAL REGULATION ; GENETIC-VARIATION ; SEQUENCING DATA ; DRUG TARGETS ; LIFE-STYLE ; GENOME ; POPULATION ; MODELS ; VARIANTS ; DATABASE |
| 收录类别 | SCI |
| 语种 | 英语 |
| 源URL | [http://ir.itp.ac.cn/handle/311006/20960]  |
| 专题 | 理论物理研究所_理论物理所1978-2010年知识产出 |
| 推荐引用方式 GB/T 7714 | Handelman, SK,Seweryn, M,Smith, RM,et al. Conditional entropy in variation-adjusted windows detects selection signatures associated with expression quantitative trait loci (eQTLs)[J]. BMC GENOMICS,2015,16(0):S8. |
| APA | Handelman, SK.,Seweryn, M.,Smith, RM.,Hartmann, K.,Wang, DX.,...&Sadee, W.(2015).Conditional entropy in variation-adjusted windows detects selection signatures associated with expression quantitative trait loci (eQTLs).BMC GENOMICS,16(0),S8. |
| MLA | Handelman, SK,et al."Conditional entropy in variation-adjusted windows detects selection signatures associated with expression quantitative trait loci (eQTLs)".BMC GENOMICS 16.0(2015):S8. |
入库方式： OAI收割
来源：理论物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。