Mechanical force-induced polymerization and depolymerization of F-actin at water/solid interfaces
文献类型：期刊论文
| 作者 | Zhang, XQ; Hu, XY; Lei, HZ; Hu, J; Zhang, Y |
| 刊名 | NANOSCALE
 |
| 出版日期 | 2016 |
| 卷号 | 8期号:11页码:6008—6013 |
| 关键词 | FOCAL ADHESIONS MOTILITY DRIVEN CELL MOTILITY FILAMENTS MICROSCOPY CYTOSKELETON NANOFIBERS NUCLEATION INTEGRINS COMPLEXES |
| ISSN号 | 2040-3364 |
| 通讯作者 | Zhang, Y (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Key Lab Interfacial Phys & Technol, Shanghai 201800, Peoples R China. |
| 英文摘要 | Actin molecules are among the three main cytoskeleton proteins of cells and undergo rapid cycling to regulate critical processes such as endocytosis, cytokinesis, cell polarity, and cell morphogenesis. Although extensive studies have been carried out on the dynamics as well as biological functions of actin polymerization and depolymerization both in vivo and in vitro, the molecular mechanisms by which cells sense and respond to mechanical signals are not fully understood. In particular, little attention has been paid to the effect of a physical force that is exerted directly on the actin cytoskeleton. In this paper, we have explored how the mechanical force affects the actin polymerization and depolymerization behaviors at water/solid interfaces using an atomic force microscope (AFM) operated in liquid. By raster scanning an AFM probe on a substrate surface with a certain load, it was found that actin monomers could polymerize into filaments without the help of actin related proteins (ARPs). Further study indicated that actin monomers were inclined to form filaments only under a small scanning load. The polymerized actin filaments would be depolymerized when the mechanical force was stronger. A possible mechanism has been suggested to explain the mechanical force induced actin polymerization. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000372245900026 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/25770]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Zhang, XQ,Hu, XY,Lei, HZ,et al. Mechanical force-induced polymerization and depolymerization of F-actin at water/solid interfaces[J]. NANOSCALE,2016,8(11):6008—6013. |
| APA | Zhang, XQ,Hu, XY,Lei, HZ,Hu, J,&Zhang, Y.(2016).Mechanical force-induced polymerization and depolymerization of F-actin at water/solid interfaces.NANOSCALE,8(11),6008—6013. |
| MLA | Zhang, XQ,et al."Mechanical force-induced polymerization and depolymerization of F-actin at water/solid interfaces".NANOSCALE 8.11(2016):6008—6013. |
入库方式： OAI收割
来源：上海应用物理研究所
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