Radiolabeling of DNA Bipyramid and Preliminary Biological Evaluation in Mice
文献类型：期刊论文
| 作者 | Li, JB; Jiang, DW; Bao, BL; He, YL; Liu, L; Wang, XM |
| 刊名 | BIOCONJUGATE CHEMISTRY
 |
| 出版日期 | 2016 |
| 卷号 | 27期号:4页码:905—910 |
| 关键词 | IN-VIVO PRECLINICAL EVALUATION NANOSTRUCTURES DELIVERY PLATFORM CANCER CELLS PET |
| ISSN号 | 1043-1802 |
| 通讯作者 | Li, JB ; Wang, XM (reprint author), Inner Mongolia Med Univ, Dept Nucl Med, Affiliated Hosp, 1 Tongdao North St, Hohhot 010050, Peoples R China. |
| 英文摘要 | Self-assembled DNA nanostructures, as a new type of nanocarriers, have shown great potential in molecular imaging probes. DNA bipyramid nanostructures (DBNs), as a classic kind of DNA nanostructure, can be precisely constructed through the unparalleled base-pairing precision of oligonucleotide strands. DBNs were prepared by self-assembly of six oligonucleotides with equal molar amounts in a single annealing step, and purified by high performance liquid chromatography (HPLC). DBNs were stable in 10% FBS as well as 80% mouse serum for at least 8 h. To prepare Tc-99m-labeled DBNs, N-hydroxysuccinimidyl S-acetylmercaptoacetyl triglycinate (Sacetyl-MAG3-NHS ester) tagged single-stranded DNA (ssDNA) was first radiolabeled with Tc-99m, and DBNs with an overhang were assembled and then hybridized with Tc-99m-ssDNA to prepare Tc-99m-labeled DNA bipyramid nanostructures (Tc-99m-MAG3-DBNs). DBNs were radiolabeled, with the radiochemical purity being over 90%. The plasma half-life of Tc-99m-MAG3-DBNs in normal KM mice was about 6 min. The biodistribution and SPECT/CT imaging were conducted with Tc-99m-MAG3-DBNs in KM mice, and both showed that Tc-99m-MAG3-DBNs mainly concentrated in the intestine, liver, and kidneys, and there was also prominent uptake in the gallbladder and bladder. We successfully obtained a new class of SPECT molecular probes based on this DNA polyhedron structure. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000374812600008 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/25822]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Li, JB,Jiang, DW,Bao, BL,et al. Radiolabeling of DNA Bipyramid and Preliminary Biological Evaluation in Mice[J]. BIOCONJUGATE CHEMISTRY,2016,27(4):905—910. |
| APA | Li, JB,Jiang, DW,Bao, BL,He, YL,Liu, L,&Wang, XM.(2016).Radiolabeling of DNA Bipyramid and Preliminary Biological Evaluation in Mice.BIOCONJUGATE CHEMISTRY,27(4),905—910. |
| MLA | Li, JB,et al."Radiolabeling of DNA Bipyramid and Preliminary Biological Evaluation in Mice".BIOCONJUGATE CHEMISTRY 27.4(2016):905—910. |
入库方式： OAI收割
来源：上海应用物理研究所
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