Structural investigation into physiological DNA phosphorothioate modification
文献类型：期刊论文
| 作者 | Lan, WX; Hu, ZP; Shen, J; Wang, CX; Jiang, F; Liu, HL; Long, DW; Liu, ML; Cao, CY |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2016 |
| 卷号 | 6期号:-页码:— |
| 关键词 | STREPTOMYCES-LIVIDANS ESCHERICHIA-COLI NMR-SPECTROSCOPY PROTEIN STABILITY BACTERIA SPECTRA SYSTEM SITE IRON |
| ISSN号 | 2045-2322 |
| 通讯作者 | Cao, CY (reprint author), Chinese Acad Sci, Shanghai Inst Organ Chem, State Key Lab Bioorgan & Nat Prod Chem, 345 Lingling Rd, Shanghai 200032, Peoples R China. |
| 英文摘要 | DNA phosphorothioate (PT) modification, with sulfur replacing a nonbridging phosphate oxygen in a sequence and stereo specific manner, is a novel physiological variation in bacteria. But what effects on DNA properties PT modification has is still unclear. To address this, we prepared three double-stranded (ds) DNA decamers, d(CG(PX)GCCGCCGA) with its complementary strand d(TCGGCG(PX)GCCG) (where X = O or S, i.e., PT-free dsDNA, [S-p, S-p]-PT dsDNA or [R-p, R-p]-PT dsDNA) located in gene of Streptomyces lividans. Their melting temperature (T-m) measurement indicates that [R-p, R-p]-PT dsDNA is most unstable. Their electron transfer potential detection presents an order of anti-oxidation properties: S-p-PT DNA > R-p-PT DNA > PT-free DNA. Their NMR structures demonstrate that PT modification doesn't change their B-form conformation. The sulfur in [R-p, R-p]-PT dsDNA locates in the major groove, with steric effects on protons in the sugar close to modification sites, resulting in its unstability, and facilitating its selectively interactions with ScoMcrA. We thought that PT modification was dialectical to the bacteria. It protects the hosting bacteria by working as antioxidant against H2O2, and acts as a marker, directing restriction enzyme observed in other hosts, like ScoMcrA, to correctly cleave the PT modified DNA, so that bacteria cannot spread and survive. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000375672600001 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/25844]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Lan, WX,Hu, ZP,Shen, J,et al. Structural investigation into physiological DNA phosphorothioate modification[J]. SCIENTIFIC REPORTS,2016,6(-):—. |
| APA | Lan, WX.,Hu, ZP.,Shen, J.,Wang, CX.,Jiang, F.,...&Cao, CY.(2016).Structural investigation into physiological DNA phosphorothioate modification.SCIENTIFIC REPORTS,6(-),—. |
| MLA | Lan, WX,et al."Structural investigation into physiological DNA phosphorothioate modification".SCIENTIFIC REPORTS 6.-(2016):—. |
入库方式： OAI收割
来源：上海应用物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。