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Endocardium Minimally Contributes to Coronary Endothelium in the Embryonic Ventricular Free Walls
文献类型：期刊论文
| 作者 | Zhang, H; Pu, WJ; Li, G; Huang, XZ; He, LJ; Tian, XY; Liu, QZ; Zhang, LB; Wu, SM; Sucov, HM |
| 刊名 | CIRCULATION RESEARCH
 |
| 出版日期 | 2016 |
| 卷号 | 118期号:12页码:1880-1880 |
| 关键词 | DEVELOPING HEART CELLS ANGIOGENESIS VASCULATURE LINEAGE MOUSE ARTERIES FORM POPULATIONS MYOCARDIUM |
| 通讯作者 | Zhou, B (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Nutr Sci, Key Lab Nutr & Metab,Grad Sch, Shanghai 200031, Peoples R China.,zhoubin@sibs.ac.cn |
| 英文摘要 | Rationale: There is persistent uncertainty regarding the developmental origins of coronary vessels, with 2 principal sources suggested as ventricular endocardium or sinus venosus (SV). These 2 proposed origins implicate fundamentally distinct mechanisms of vessel formation. Resolution of this controversy is critical for deciphering the programs that result in the formation of coronary vessels and has implications for research on therapeutic angiogenesis. Objective: To resolve the controversy over the developmental origin of coronary vessels. Methods and Results: We first generated nuclear factor of activated T cells (Nfatc1)-Cre and Nfatc1-Dre lineage tracers for endocardium labeling. We found that Nfatc1 recombinases also label a significant portion of SV endothelial cells in addition to endocardium. Therefore, restricted endocardial lineage tracing requires a specific marker that distinguishes endocardium from SV. By single-cell gene expression analysis, we identified a novel endocardial gene natriuretic peptide receptor 3 (Npr3). Npr3 is expressed in the entirety of the endocardium but not in the SV. Genetic lineage tracing based on Npr3-CreER showed that endocardium contributes to a minority of coronary vessels in the free walls of embryonic heart. Intersectional genetic lineage tracing experiments demonstrated that endocardium minimally contributes to coronary endothelium in the embryonic ventricular free walls. Conclusions: Our study suggested that SV, but not endocardium, is the major origin for coronary endothelium in the embryonic ventricular free walls. This work thus resolves the recent controversy over the developmental origin of coronary endothelium, providing the basis for studying coronary vessel formation and regeneration after injury. |
| WOS标题词 | Cardiovascular System & Cardiology ; Hematology |
| 学科主题 | Cardiac & Cardiovascular Systems; Hematology; Peripheral Vascular Disease |
| 语种 | 英语 |
| WOS记录号 | WOS:000377885100009 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/4018]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Zhang, H,Pu, WJ,Li, G,et al. Endocardium Minimally Contributes to Coronary Endothelium in the Embryonic Ventricular Free Walls[J]. CIRCULATION RESEARCH,2016,118(12):1880-1880. |
| APA | Zhang, H.,Pu, WJ.,Li, G.,Huang, XZ.,He, LJ.,...&Zhou, B.(2016).Endocardium Minimally Contributes to Coronary Endothelium in the Embryonic Ventricular Free Walls.CIRCULATION RESEARCH,118(12),1880-1880. |
| MLA | Zhang, H,et al."Endocardium Minimally Contributes to Coronary Endothelium in the Embryonic Ventricular Free Walls".CIRCULATION RESEARCH 118.12(2016):1880-1880. |
入库方式： OAI收割
来源：上海神经科学研究所
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