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Long-Range Chromosome Interactions Mediated by Cohesin Shape Circadian Gene Expression
文献类型：期刊论文
| 作者 | Xu, YC; Guo, WM; Li, P; Zhang, Y; Zhao, M; Fan, ZH; Zhao, ZH; Yan, J |
| 刊名 | PLOS GENETICS
 |
| 出版日期 | 2016 |
| 卷号 | 12期号:5页码:e1005992 |
| 关键词 | CELL IDENTITY GENES GENOME-WIDE CHROMATIN INTERACTOME SUPER-ENHANCERS TRANSCRIPTION CTCF BINDING CLOCK ORGANIZATION DIFFERENTIATION |
| 通讯作者 | Yan, J (reprint author), Chinese Acad Sci, CAS MPG Partner Inst Computat Biol, Shanghai Inst Biol Sci, Shanghai, Peoples R China. ; Yan, J (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Neurosci, State Key Lab Neurosci,CAS Ctr Excellence Brain S, Shanghai, Peoples R China.,junyan@picb.ac.cn |
| 英文摘要 | Mammalian circadian rhythm is established by the negative feedback loops consisting of a set of clock genes, which lead to the circadian expression of thousands of downstream genes in vivo. As genome-wide transcription is organized under the high-order chromosome structure, it is largely uncharted how circadian gene expression is influenced by chromosome architecture. We focus on the function of chromatin structure proteins cohesin as well as CTCF (CCCTC-binding factor) in circadian rhythm. Using circular chromosome conformation capture sequencing, we systematically examined the interacting loci of a Bmal1-bound super-enhancer upstream of a clock gene Nr1d1 in mouse liver. These interactions are largely stable in the circadian cycle and cohesin binding sites are enriched in the interactome. Global analysis showed that cohesin-CTCF co-binding sites tend to insulate the phases of circadian oscillating genes while cohesin-non-CTCF sites are associated with high circadian rhythmicity of transcription. A model integrating the effects of cohesin and CTCF markedly improved the mechanistic understanding of circadian gene expression. Further experiments in cohesin knockout cells demonstrated that cohesin is required at least in part for driving the circadian gene expression by facilitating the enhancer-promoter looping. This study provided a novel insight into the relationship between circadian transcriptome and the high-order chromosome structure. |
| WOS标题词 | Genetics & Heredity |
| 学科主题 | Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000377197100006 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/4024]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Xu, YC,Guo, WM,Li, P,et al. Long-Range Chromosome Interactions Mediated by Cohesin Shape Circadian Gene Expression[J]. PLOS GENETICS,2016,12(5):e1005992. |
| APA | Xu, YC.,Guo, WM.,Li, P.,Zhang, Y.,Zhao, M.,...&Yan, J.(2016).Long-Range Chromosome Interactions Mediated by Cohesin Shape Circadian Gene Expression.PLOS GENETICS,12(5),e1005992. |
| MLA | Xu, YC,et al."Long-Range Chromosome Interactions Mediated by Cohesin Shape Circadian Gene Expression".PLOS GENETICS 12.5(2016):e1005992. |
入库方式： OAI收割
来源：上海神经科学研究所
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