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CRASP: CFP reconstitution across synaptic partners
文献类型：期刊论文
| 作者 | Li, YM; Guo, AK; Li, H |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2016 |
| 卷号 | 469期号:3页码:352-356 |
| 关键词 | GREEN FLUORESCENT PROTEIN MUSHROOM BODY DROSOPHILA CIRCUIT REPRESENTATION NEURONS GRASP MAP |
| 通讯作者 | Li, H (reprint author), Chinese Acad Sci, Inst Neurosci, State Key Lab Neurosci, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China.,hli@ion.ac.cn |
| 英文摘要 | Mapping the pattern of connectivity between neurons is widely regarded to be critical for understanding the nervous system. GRASP (GFP reconstitution across synaptic partners) has been used as a promising method for mapping neuronal connectivity, but is currently available in the green color only, limiting its potential applications. Here we demonstrate CRASP (CFP reconstitution across synaptic partners), a cyan colored version of GRASP. We validated the system in HEK 293T cells, and generated transgenic Drosophila lines to show that the system could reliably detect neuronal contacts in the brain. Furthermore, we showed that the CRASP signal could be selectively amplified using standard immunohistochemistry methods. The CRASP system adds to the toolkit available to researchers for mapping neuronal connectivity, and substantially expands the potential application of GRASP-like strategies. (C) 2015 Elsevier Inc. All rights reserved. |
| WOS标题词 | Biochemistry & Molecular Biology ; Biophysics |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000369352800004 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/4045]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Li, YM,Guo, AK,Li, H. CRASP: CFP reconstitution across synaptic partners[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2016,469(3):352-356. |
| APA | Li, YM,Guo, AK,&Li, H.(2016).CRASP: CFP reconstitution across synaptic partners.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,469(3),352-356. |
| MLA | Li, YM,et al."CRASP: CFP reconstitution across synaptic partners".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 469.3(2016):352-356. |
入库方式： OAI收割
来源：上海神经科学研究所
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