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Somatosensory neuron types identified by high-coverage single-cell RNA-sequencing and functional heterogeneity
文献类型：期刊论文
| 作者 | Li, CL; Li, KC; Wu, D; Chen, Y; Luo, H; Zhao, JR; Wang, SS; Sun, MM; Lu, YJ; Zhong, YQ |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2016 |
| 卷号 | 26期号:1页码:83-102 |
| 关键词 | NOCICEPTIVE SENSORY NEURONS NATRIURETIC PEPTIDE NERVE INJURY DORSAL-ROOT ION-CHANNEL PAIN SEQ EXPRESSION RECEPTOR MICE |
| 通讯作者 | Zhang, X (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, CAS Ctr Excellence Brain Sci, Inst Neurosci, Shanghai 20031, Peoples R China.,xu.zhang@ion.ac.cn |
| 英文摘要 | Sensory neurons are distinguished by distinct signaling networks and receptive characteristics. Thus, sensory neuron types can be defined by linking transcriptome-based neuron typing with the sensory phenotypes. Here we classify somatosensory neurons of the mouse dorsal root ganglion (DRG) by high-coverage single-cell RNA-sequencing (10 950 +/- 1 218 genes per neuron) and neuron size-based hierarchical clustering. Moreover, single DRG neurons responding to cutaneous stimuli are recorded using an in vivo whole-cell patch clamp technique and classified by neuron-type genetic markers. Small diameter DRG neurons are classified into one type of low-threshold mechanoreceptor and five types of mechanoheat nociceptors (MHNs). Each of the MHN types is further categorized into two subtypes. Large DRG neurons are categorized into four types, including neurexophilin 1-expressing MHNs and mechanical nociceptors (MNs) expressing BAI1-associated protein 2-like 1 (Baiap2l1). Mechanoreceptors expressing trafficking protein particle complex 3-like and Baiap2l1-marked MNs are subdivided into two subtypes each. These results provide a new system for cataloging somatosensory neurons and their transcriptome databases. |
| 学科主题 | Cell Biology |
| WOS标题词 | Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000367615200009 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/4049]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Li, CL,Li, KC,Wu, D,et al. Somatosensory neuron types identified by high-coverage single-cell RNA-sequencing and functional heterogeneity[J]. CELL RESEARCH,2016,26(1):83-102. |
| APA | Li, CL.,Li, KC.,Wu, D.,Chen, Y.,Luo, H.,...&Zhang, X.(2016).Somatosensory neuron types identified by high-coverage single-cell RNA-sequencing and functional heterogeneity.CELL RESEARCH,26(1),83-102. |
| MLA | Li, CL,et al."Somatosensory neuron types identified by high-coverage single-cell RNA-sequencing and functional heterogeneity".CELL RESEARCH 26.1(2016):83-102. |
入库方式： OAI收割
来源：上海神经科学研究所
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