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Genetic lineage tracing identifies in situ Kit-expressing cardiomyocytes
文献类型：期刊论文
| 作者 | Liu, QZ; Yang, R; Huang, XZ; Zhang, H; He, LJ; Zhang, LB; Tian, XY; Nie, Y; Hu, SS; Yan, Y |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2016 |
| 卷号 | 26期号:1页码:119-130 |
| 关键词 | CARDIAC STEM-CELLS CRE KNOCK-INS C-KIT ISCHEMIC CARDIOMYOPATHY HEART ADULT REGENERATION REPAIR PRECURSORS PITFALLS |
| 通讯作者 | Zhou, B (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Key Lab Nutr & Metab, Inst Nutr Sci,Grad Sch, Shanghai 200031, Peoples R China.,zhoubin@sibs.ac.cn |
| 英文摘要 | Cardiac cells marked by c-Kit or Kit, dubbed cardiac stem cells (CSCs), are in clinical trials to investigate their ability to stimulate cardiac regeneration and repair. These studies were initially motivated by the purported cardiogenic activity of these cells. Recent lineage tracing studies using Kit promoter to drive expression of the inducible Cre recombinase showed that these CSCs had highly limited cardiogenic activity, inadequate to support efficient cardiac repair. Here we reassess the lineage tracing data by investigating the identity of cells immediately after Cre labeling. Our instant lineage tracing approach identifies Kit-expressing cardiomyocytes, which are labeled immediately after tamoxifen induction. In combination with long-term lineage tracing experiments, these data reveal that the large majority of long-term labeled cardiomyocytes are pre-existing Kit-expressing cardiomyocytes rather than cardiomyocytes formed de novo from CSCs. This study presents a new interpretation for the contribution of Kit(+) cells to cardiomyocytes and shows that Kit genetic lineage tracing over-estimates the cardiogenic activity of Kit(+) CSCs. |
| WOS标题词 | Cell Biology |
| 学科主题 | Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000367615200011 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/4050]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Liu, QZ,Yang, R,Huang, XZ,et al. Genetic lineage tracing identifies in situ Kit-expressing cardiomyocytes[J]. CELL RESEARCH,2016,26(1):119-130. |
| APA | Liu, QZ.,Yang, R.,Huang, XZ.,Zhang, H.,He, LJ.,...&Zhou, B.(2016).Genetic lineage tracing identifies in situ Kit-expressing cardiomyocytes.CELL RESEARCH,26(1),119-130. |
| MLA | Liu, QZ,et al."Genetic lineage tracing identifies in situ Kit-expressing cardiomyocytes".CELL RESEARCH 26.1(2016):119-130. |
入库方式： OAI收割
来源：上海神经科学研究所
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