Characterization of a cold-adapted and salt-tolerant exo-chitinase (ChiC) from Pseudoalteromonas sp DL-6
文献类型：期刊论文
| 作者 | Wang, Xiaohui1,2,3; Chi, Naiyu3; Bai, Fengwu1; Du, Yuguang4; Zhao, Yong2; Yin, Heng2 |
| 刊名 | EXTREMOPHILES
 |
| 出版日期 | 2016-03-01 |
| 卷号 | 20期号:2页码:167-176 |
| 关键词 | Chitin Exochitinase Cold-adapted Salt-tolerant Pseudoalteromonas sp. |
| ISSN号 | 1431-0651 |
| 英文摘要 | We have previously reported a non-processive endo-type chitinase, ChiA, from a newly isolated marine psychrophilic bacterium, Pseudoalteromonas sp. DL-6. In this study, a processive exo-type chitinase, ChiC, was cloned from the same bacterium and characterized in detail. ChiC could hydrolyze crystalline chitin into (GlcNAc)(2) as the only observed product. It exhibited high catalytic activity even at low temperatures, e.g. close to 0 degrees C, or in the presence of 5 M NaCl, suggesting that ChiC was a cold-adapted and highly salt-tolerant chitinase. ChiC could also hydrolyze other substrates, including chitosan and Avicel, indicating its broad substrate specificity. Sequence features indicated that ChiC was a multi-domain protein having a deep substrate-binding groove that was regarded as characteristic of processive exo-chitinases. Enzymatic hydrolysis of chitin by ChiC could be remarkably boosted in the presence of ChiA, suggesting the synergy of ChiC and ChiA. This work provided a new evidence to prove that marine psychrophilic bacteria utilized a synergistic enzyme system to degrade recalcitrant chitin. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Microbiology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Microbiology |
| 关键词[WOS] | MARINE PSYCHROPHILIC BACTERIUM ; SP STRAIN O-7 ; SERRATIA-MARCESCENS ; RECALCITRANT POLYSACCHARIDES ; BIOCHEMICAL-CHARACTERIZATION ; MORITELLA-MARINA ; BINDING DOMAIN ; BETA-CHITIN ; DEGRADATION ; PROCESSIVITY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000374832600006 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/21037]  |
| 专题 | 过程工程研究所_管理及支撑部门 |
| 作者单位 | 1.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116024, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Nat Prod & Glycobiotechnol Res Grp, Dalian 116023, Peoples R China 3.Dalian Univ, Sch Life Sci & Biotechnol, Dalian 116622, Peoples R China 4.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xiaohui,Chi, Naiyu,Bai, Fengwu,et al. Characterization of a cold-adapted and salt-tolerant exo-chitinase (ChiC) from Pseudoalteromonas sp DL-6[J]. EXTREMOPHILES,2016,20(2):167-176. |
| APA | Wang, Xiaohui,Chi, Naiyu,Bai, Fengwu,Du, Yuguang,Zhao, Yong,&Yin, Heng.(2016).Characterization of a cold-adapted and salt-tolerant exo-chitinase (ChiC) from Pseudoalteromonas sp DL-6.EXTREMOPHILES,20(2),167-176. |
| MLA | Wang, Xiaohui,et al."Characterization of a cold-adapted and salt-tolerant exo-chitinase (ChiC) from Pseudoalteromonas sp DL-6".EXTREMOPHILES 20.2(2016):167-176. |
入库方式： OAI收割
来源：过程工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。