Efficient production of succinic acid from Palmaria palmata hydrolysate by metabolically engineered Escherichia coli
文献类型：期刊论文
| 作者 | Olajuyin, Ayobami Matthew1,2; Yang, Maohua1; Liu, Yilan1; Mu, Tingzhen1,2; Tian, Jiangnan1,2; Adaramoye, Oluwatosin Adekunle3; Xing, Jianmin1 |
| 刊名 | BIORESOURCE TECHNOLOGY
 |
| 出版日期 | 2016-08-01 |
| 卷号 | 214期号:AUG页码:653-659 |
| ISSN号 | 0960-8524 |
| 关键词 | Palmaria palmata Escherichia coli Succinic acid Galactose Fermentation |
| 英文摘要 | Succinic acid, a C4 dicarboxylic acid is used in many fields such as food, agriculture, pharmaceutical and polymer industries. In this study, microbial production of succinic acid from Palmaria palmata was investigated for the first time. In engineered Escherichia coli KLPPP, lactate dehydrogenase, pyruvate formate lyase, phosphotransacetylase-acetate kinase and pyruvate oxidase genes were deleted while phosphoenolpyruvate carboxykinase was overexpressed. The recombinant exhibited higher molar yield of succinic acid on galactose (1.20 +/- 0.02 mol/mol) than glucose (0.48 +/- 0.03 mol/mol). The concentration and molar yield of succinic acid were 22.40 +/- 0.12 g/L and 1.13 +/- 0.02 mol/mol total sugar respectively after 72 h dual phase fermentation from P. palmata hydrolysate which composed of glucose (12.57 +/- 0.17 g/L) and galactose (18.03 +/- 0.10 g/L). The results demonstrate that P. palmata red macroalgae biomass represents a novel and an economically alternative feedstock for biochemicals production. (C) 2016 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 研究领域[WOS] | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 关键词[WOS] | ANAEROBIOSPIRILLUM-SUCCINICIPRODUCENS ; BIOFUEL PRODUCTION ; DIAUXIC GROWTH ; FERMENTATION ; MACROALGAE ; GALACTOSE ; GLUCOSE ; STRAIN ; PATHWAYS ; MUTATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000377366900083 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/20957]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Univ Ibadan, Dept Biochem, Fac Basic Med Sci, Ibadan, Nigeria |
| 推荐引用方式 GB/T 7714 | Olajuyin, Ayobami Matthew,Yang, Maohua,Liu, Yilan,et al. Efficient production of succinic acid from Palmaria palmata hydrolysate by metabolically engineered Escherichia coli[J]. BIORESOURCE TECHNOLOGY,2016,214(AUG):653-659. |
| APA | Olajuyin, Ayobami Matthew.,Yang, Maohua.,Liu, Yilan.,Mu, Tingzhen.,Tian, Jiangnan.,...&Xing, Jianmin.(2016).Efficient production of succinic acid from Palmaria palmata hydrolysate by metabolically engineered Escherichia coli.BIORESOURCE TECHNOLOGY,214(AUG),653-659. |
| MLA | Olajuyin, Ayobami Matthew,et al."Efficient production of succinic acid from Palmaria palmata hydrolysate by metabolically engineered Escherichia coli".BIORESOURCE TECHNOLOGY 214.AUG(2016):653-659. |
入库方式： OAI收割
来源：过程工程研究所
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