Pathogen-Mimicking Polymeric Nanoparticles based on Dopamine Polymerization as Vaccines Adjuvants Induce Robust Humoral and Cellular Immune Responses
文献类型：期刊论文
| 作者 | Liu, Qi1,2; Jia, Jilei1,2; Yang, Tingyuan1; Fan, Qingze1,2; Wang, Lianyan1; Ma, Guanghui1,3 |
| 刊名 | SMALL
 |
| 出版日期 | 2016-04-06 |
| 卷号 | 12期号:13页码:1744-1757 |
| 关键词 | dopamine polymerization pathogen-mimicking polymeric nanoparticles surface modification vaccine delivery and adjuvant systems |
| ISSN号 | 1613-6810 |
| 英文摘要 | Aiming to enhance the immunogenicity of subunit vaccines, a novel antigen delivery and adjuvant system based on dopamine polymerization on the surface of poly(d,l-lactic-glycolic-acid) nanoparticles (NPs) with multiple mechanisms of immunity enhancement is developed. The mussel-inspired biomimetic polydopamine (pD) not only serves as a coating to NPs but also functionalizes NP surfaces. The method is facile and mild including simple incubation of the preformed NPs in the weak alkaline dopamine solution, and incorporation of hepatitis B surface antigen and TLR9 agonist unmethylated cytosine-guanine (CpG) motif with the pD surface. The as-constructed NPs possess pathogen-mimicking manners owing to their size, shape, and surface molecular immune-activating properties given by CpG. The biocompatibility and biosafety of these pathogen-mimicking NPs are confirmed using bone marrow-derived dendritic cells. Pathogen-mimicking NPs hold great potential as vaccine delivery and adjuvant system due to their ability to: 1) enhance cytokine secretion and immune cell recruitment at the injection site; 2) significantly activate and maturate dendritic cells; 3) induce stronger humoral and cellular immune responses in vivo. Furthermore, this simple and versatile dopamine polymerization method can be applicable to endow NPs with characteristics to mimic pathogen structure and function, and manipulate NPs for the generation of efficacious vaccine adjuvants. |
| WOS标题词 | Science & Technology ; Physical Sciences ; Technology |
| 类目[WOS] | Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter |
| 研究领域[WOS] | Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics |
| 关键词[WOS] | DENDRITIC CELLS ; INNATE IMMUNITY ; IN-VIVO ; SURFACE MODIFICATION ; BACTERIAL-DNA ; HIV-1 P24 ; CPG DNA ; DELIVERY ; POLYDOPAMINE ; ANTIGEN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000373733200010 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/20982]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Natl Key Lab Biochem Engn, PLA Key Lab Biopharmaceut Prod & Formulat Engn, Inst Proc Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chem Sci & Engn Tianjin, Collaborat Innovat Ctr, Tianjin 300072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Qi,Jia, Jilei,Yang, Tingyuan,et al. Pathogen-Mimicking Polymeric Nanoparticles based on Dopamine Polymerization as Vaccines Adjuvants Induce Robust Humoral and Cellular Immune Responses[J]. SMALL,2016,12(13):1744-1757. |
| APA | Liu, Qi,Jia, Jilei,Yang, Tingyuan,Fan, Qingze,Wang, Lianyan,&Ma, Guanghui.(2016).Pathogen-Mimicking Polymeric Nanoparticles based on Dopamine Polymerization as Vaccines Adjuvants Induce Robust Humoral and Cellular Immune Responses.SMALL,12(13),1744-1757. |
| MLA | Liu, Qi,et al."Pathogen-Mimicking Polymeric Nanoparticles based on Dopamine Polymerization as Vaccines Adjuvants Induce Robust Humoral and Cellular Immune Responses".SMALL 12.13(2016):1744-1757. |
入库方式： OAI收割
来源：过程工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。