Functional hydrophilic polystyrene beads with uniformly size and high cross-linking degree facilitated rapid separation of exenatide
文献类型：期刊论文
| 作者 | Li, Qiang1; Zhao, Lan1; Zhang, Rongyue2; Huang, Yongdong1; Zhang, Yan1; Zhang, Kun1,3; Wu, Xuexing1; Zhang, Zhigang3; Gong, Fangling1; Su, Zhiguo1 |
| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
 |
| 出版日期 | 2016-04-01 |
| 卷号 | 1017期号:APR页码:129-135 |
| 关键词 | Polystyrene microspheres Hydrophilicity Anion-exchange Peptide separation |
| ISSN号 | 1570-0232 |
| 英文摘要 | A high cross-linking polystyrene(PSt)-based anion-exchange material with uniformly size, high ion exchange capacity, and high hydrophilicity was synthesized by a novel surface functionalization approach in this study. Uniformly sized PSt microspheres were prepared by the membrane emulsion polymerization strategy, and then modified by (1) conversing resid ual surface vinyl groups to epoxy groups followed by quaternization, and (2) decorating aromatic ring matrix including nitration, reduction and attachment of glycidyltrimethylammonium chloride. The 3-D morphology and porous features of microspheres were observed by scanning electron microscopy (SEM) and atomic force microscopy (AFM). The surface of the modified PSt became roughness but the particle size remained same. Meanwhile, FT-IR spectra and laser scanning confocal microscope (LCSM) indicated that the modification groups had been successfully covalently coated onto the PSt microspheres. Modified PSt microspheres showed greatly improved hydrophilicity and biocompatibility with 0.387 mmol/mL ion exchange capacity (IEC). In the application evaluation procedure, exenatide can be purified from 42.9% (peptide crudes) to 88.6% by modified PSt column with 97.1% recovery yield. This modified PSt microspheres had a large potential in application for efficient separation of peptides. (C) 2016 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | FRIEDEL-CRAFTS REACTION ; EXCHANGE CHROMATOGRAPHY ; GRAM SCALE ; ANALOG ; PURIFICATION ; PARTICLES ; EXENDIN-4 ; CATALYSTS ; PROTEINS ; CHLORIDE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000373547200016 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/20985]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Beijing Inst Petrochem Technol, Dept Chem Engn, Beijing 102617, Peoples R China 3.Hebei Normal Univ Sci & Technol, Qinhuangdao 066004, Hebei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Qiang,Zhao, Lan,Zhang, Rongyue,et al. Functional hydrophilic polystyrene beads with uniformly size and high cross-linking degree facilitated rapid separation of exenatide[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2016,1017(APR):129-135. |
| APA | Li, Qiang.,Zhao, Lan.,Zhang, Rongyue.,Huang, Yongdong.,Zhang, Yan.,...&Ma, Guanghui.(2016).Functional hydrophilic polystyrene beads with uniformly size and high cross-linking degree facilitated rapid separation of exenatide.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1017(APR),129-135. |
| MLA | Li, Qiang,et al."Functional hydrophilic polystyrene beads with uniformly size and high cross-linking degree facilitated rapid separation of exenatide".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1017.APR(2016):129-135. |
入库方式： OAI收割
来源：过程工程研究所
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