Potent Antigen-Adjuvant Delivery System by Conjugation of Mycobacterium tuberculosis Ag85B-HspX Fusion Protein with Arabinogalactan-Poly(I:C) Conjugate
文献类型：期刊论文
| 作者 | Huang, Qingrui1,2; Yu, Weili1,2; Hu, Tao1 |
| 刊名 | BIOCONJUGATE CHEMISTRY
 |
| 出版日期 | 2016-04-01 |
| 卷号 | 27期号:4页码:1165-1174 |
| ISSN号 | 1043-1802 |
| 英文摘要 | Protein-based vaccine is promising to improve or replace Mycobacterium bovis BCG vaccine for its specificity, safety, and easy production. However, protein-based vaccine calls for potent adjuvants and improved delivery systems to protect against Mycobacterium tuberculosis. Poly(I:C) is one of the most potent pathogen-associated molecular patterns that signals primarily via TLR3. Arabinogalactan (AG) is a biocompatible polysaccharide that can increase splenocyte proliferation and stimulate macrophages. The AG-poly(I:C) conjugate (AG-P) showed an adjuvant potency through a synergistic interaction of AG and poly(I:C). Ag85B and HspX are two important virulent protein antigens of Mycobacterium tuberculosis and Ag85B-HspX fusion protein (AH) was prepared. An antigen-adjuvant delivery system (AH-AG-P) was developed by conjugation of AH with AG-P to ensure that both AH and AG-P reach the APCs simultaneously. AH-AG-P elicited high AH-specific IgG titers and stimulated lymphocyte proliferation. AH-AG-P provoked the secretion of Th1-type cytokines (TNF-alpha, IFN-gamma, and IL-2) and Th2-type cytokines (IL-4 and IL-10). Pharmacokinetics revealed that conjugation with AG-P could prolong the serum exposure of AH to the immune system. Pharmacodynamics suggested that conjugation with AG-P led to a rapid and intense production of AH-specific IgG. Accordingly, conjugation with AG-P could promote a robust cellular and humoral immune response to AH. Thus, conjugation of AH with a potent adjuvant AG-P is an effective strategy to develop an efficacious protein-based vaccine against Mycobacterium tuberculosis. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary ; Chemistry, Organic |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | RESISTANT TUBERCULOSIS ; MULTIDRUG-RESISTANT ; VACCINE DEVELOPMENT ; IMMUNOGENICITY ; POLYSACCHARIDE ; EFFICACY ; IMPROVES ; IMMUNE ; MODEL ; TIME |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000374812600034 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/21012]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Qingrui,Yu, Weili,Hu, Tao. Potent Antigen-Adjuvant Delivery System by Conjugation of Mycobacterium tuberculosis Ag85B-HspX Fusion Protein with Arabinogalactan-Poly(I:C) Conjugate[J]. BIOCONJUGATE CHEMISTRY,2016,27(4):1165-1174. |
| APA | Huang, Qingrui,Yu, Weili,&Hu, Tao.(2016).Potent Antigen-Adjuvant Delivery System by Conjugation of Mycobacterium tuberculosis Ag85B-HspX Fusion Protein with Arabinogalactan-Poly(I:C) Conjugate.BIOCONJUGATE CHEMISTRY,27(4),1165-1174. |
| MLA | Huang, Qingrui,et al."Potent Antigen-Adjuvant Delivery System by Conjugation of Mycobacterium tuberculosis Ag85B-HspX Fusion Protein with Arabinogalactan-Poly(I:C) Conjugate".BIOCONJUGATE CHEMISTRY 27.4(2016):1165-1174. |
入库方式： OAI收割
来源：过程工程研究所
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