Towards A Deeper Understanding of the Interfacial Adsorption of Enzyme Molecules in Gigaporous Polymeric Microspheres
文献类型：期刊论文
| 作者 | Wang, Weichen1,2; Zhou, Weiqing1; Wei, Wei1; Li, Juan1; Hao, Dongxia1; Su, Zhiguo1; Ma, Guanghui1 |
| 刊名 | POLYMERS
 |
| 出版日期 | 2016-04-01 |
| 卷号 | 8期号:4页码:116 |
| 关键词 | lipase adsorption gigaporous mesoporous microsphere QCM-D |
| ISSN号 | 2073-4360 |
| 英文摘要 | Compared with the one immobilized in the conventional mesoporous microspheres, the enzyme immobilized in gigaporous microspheres showed much higher activity and better stability. To gain a deeper understanding, we herein selected lipase as a prototype to comparatively analyze the adsorption behavior of lipase at interfaces in gigaporous and mesoporous polystyrene microspheres at very low lipase concentration, and further compared with the adsorption on a completely flat surface (a chip). Owing to the limited space of narrow pores, lipase molecules were inclined to be adsorbed as a monolayer in mesoporous microspheres. During this process, the interaction between lipase molecules and the interface was stronger, which could result in the structural change of lipase molecular and compromised specific activity. In addition to monolayer adsorption, more multilayer adsorption of enzyme molecules also occurred in gigaporous microspheres. Besides the adsorption state, the pore curvature also affected the lipase adsorption. Due to the multilayer adsorption, the excellent mass transfer properties for the substrate and the product in the large pores, and the small pore curvature, lipase immobilized in gigaporous microspheres showed better behaviors. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Polymer Science |
| 研究领域[WOS] | Polymer Science |
| 关键词[WOS] | QUARTZ-CRYSTAL MICROBALANCE ; CANDIDA-RUGOSA LIPASE ; PORE-SIZE ; IMMOBILIZED ENZYMES ; MESOPOROUS SILICA ; POLYESTER SYNTHESIS ; CATALYTIC-ACTIVITY ; PARTICLE-SIZE ; B ADSORPTION ; ANTARCTICA |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000375159900021 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/21017]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Weichen,Zhou, Weiqing,Wei, Wei,et al. Towards A Deeper Understanding of the Interfacial Adsorption of Enzyme Molecules in Gigaporous Polymeric Microspheres[J]. POLYMERS,2016,8(4):116. |
| APA | Wang, Weichen.,Zhou, Weiqing.,Wei, Wei.,Li, Juan.,Hao, Dongxia.,...&Ma, Guanghui.(2016).Towards A Deeper Understanding of the Interfacial Adsorption of Enzyme Molecules in Gigaporous Polymeric Microspheres.POLYMERS,8(4),116. |
| MLA | Wang, Weichen,et al."Towards A Deeper Understanding of the Interfacial Adsorption of Enzyme Molecules in Gigaporous Polymeric Microspheres".POLYMERS 8.4(2016):116. |
入库方式： OAI收割
来源：过程工程研究所
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