Effects of mixed carbon sources on galactose and mannose content of exopolysaccharides and related enzyme activities in Ganoderma lucidum
文献类型：期刊论文
| 作者 | Peng, Lin1,4; Li, Jie1,4; Liu, Ying3; Xu, Zhenghong1; Wu, Jian-Yong2; Ding, Zhongyang1,4; Gu, Zhenghua1,4; Zhang, Liang1,4; Shi, Guiyang1,4 |
| 刊名 | RSC ADVANCES
 |
| 出版日期 | 2016 |
| 卷号 | 6期号:45页码:39284-39291 |
| ISSN号 | 2046-2069 |
| 英文摘要 | This work investigated the effects of mixed carbon sources on the monosaccharide composition of exopolysaccharides (EPS) and activity of related enzymes, phosphoglucose isomerase (PGI), alpha-phosphoglucomutase (PGM), phosphomannose isomerase (PMI) and GDP-D-Man pyrophosphorylase (GMP), in the EPS biosynthesis by Ganoderma lucidum. Combinations of two carbon sources, glucose (Glc) + mannose (Man) and Glc + galactose (Gal), were applied at various mass ratios in the liquid culture of G. lucidum. The results showed that Glc, Gal, and Man were the major monosaccharides in G. lucidum EPS, and the combined mole percentages of monosaccharides were >80%, the same as those in the carbon source mixture. Activities of PGI, PMI, and GMP were correlated with Man mole percentage in EPSs and were enhanced by increasing the proportion of Gal for the carbon source Glc + Gal. PGM activity was correlated with Gal mole percentage and was reduced by increasing the Man proportion for Glc + Man. The expression of four genes encoding PGI, PMI, GMP and PGM reached peak levels on day 6 of culture, whereas the enzyme activities increased steadily from day 3 to 8. The monosaccharide composition of G. lucidum EPS for various mixed carbon source conditions thus appeared to be controlled by the translational level of genes encoding PGM, PGI, PMI, and GMP. These findings will be helpful to control the monosaccharide composition for desired biological activity of EPS produced by G. lucidum fermentation. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Multidisciplinary |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | MONOSACCHARIDE COMPOSITION ; POLYSACCHARIDE ; PATHWAYS ; BIOSYNTHESIS ; EXTRACTION ; CULTURE ; PROTEIN ; STRAIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000374972800096 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/21043]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Jiangnan Univ, Sch Biotechnol, Minist Educ, Key Lab Carbohydrate Chem & Biotechnol, Wuxi 214122, Peoples R China 2.Hong Kong Polytech Univ, Dept Appl Biol & Chem Technol, Kowloon, Hong Kong, Peoples R China 3.Chinese Acad Sci, Inst Proc Engn, Lab Separat Sci & Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 4.Jiangnan Univ, Natl Engn Lab Cereal Fermentat Technol, Wuxi 214122, Peoples R China |
| 推荐引用方式 GB/T 7714 | Peng, Lin,Li, Jie,Liu, Ying,et al. Effects of mixed carbon sources on galactose and mannose content of exopolysaccharides and related enzyme activities in Ganoderma lucidum[J]. RSC ADVANCES,2016,6(45):39284-39291. |
| APA | Peng, Lin.,Li, Jie.,Liu, Ying.,Xu, Zhenghong.,Wu, Jian-Yong.,...&Shi, Guiyang.(2016).Effects of mixed carbon sources on galactose and mannose content of exopolysaccharides and related enzyme activities in Ganoderma lucidum.RSC ADVANCES,6(45),39284-39291. |
| MLA | Peng, Lin,et al."Effects of mixed carbon sources on galactose and mannose content of exopolysaccharides and related enzyme activities in Ganoderma lucidum".RSC ADVANCES 6.45(2016):39284-39291. |
入库方式： OAI收割
来源：过程工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。