Polydopamine meets porous membrane: A versatile platform for facile preparation of membrane adsorbers
文献类型：期刊论文
| 作者 | Fan, Jinxin1,2; Luo, Jianquan1; Chen, Xiangrong1; Wan, Yinhua1 |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2016-05-27 |
| 卷号 | 1448期号:MAY页码:121-126 |
| 关键词 | Membrane adsorber Membrane chromatography Ion-exchange Hydrophobic interaction Affinity membrane adsorber |
| ISSN号 | 0021-9673 |
| 英文摘要 | Polydopamine, as an intermediate layer coated on PES membrane, was applied to fabricate various membrane adsorbers. Anion-exchange, hydrophobic interaction and affinity membrane adsorbers prepared by this facile method exhibited a high selectivity in fractionation of IgG (immunoglobulin)/HSA (human serum albumin) mixture. The anion-exchange membrane adsorber containing polyethylenimine (PEI) improved the HSA purity from 17.7% to 96.7%; The hydrophobic interaction membrane adsorber with Dodecyl mercaptan (DDM) as ligand obtained an IgG purity of 94.6%; Histidine attached affinity membrane chromatography achieved nearly a 100% purity of IgG. The present work indicated that the polydopamine layer not only activated membrane surface to attach various adsorptive ligands under the mild condition, but also reduced non-specific adsorption. Due to the versatile conjunction function, this facile mussel-inspired coating is also promising for the preparation of diverse membrane adsorbers. (C) 2016 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | PROTEIN SEPARATION ; SURFACE ; PURIFICATION ; POLYMER ; CHROMATOGRAPHY ; PERFORMANCE ; CAPACITY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000376211600015 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/21073]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fan, Jinxin,Luo, Jianquan,Chen, Xiangrong,et al. Polydopamine meets porous membrane: A versatile platform for facile preparation of membrane adsorbers[J]. JOURNAL OF CHROMATOGRAPHY A,2016,1448(MAY):121-126. |
| APA | Fan, Jinxin,Luo, Jianquan,Chen, Xiangrong,&Wan, Yinhua.(2016).Polydopamine meets porous membrane: A versatile platform for facile preparation of membrane adsorbers.JOURNAL OF CHROMATOGRAPHY A,1448(MAY),121-126. |
| MLA | Fan, Jinxin,et al."Polydopamine meets porous membrane: A versatile platform for facile preparation of membrane adsorbers".JOURNAL OF CHROMATOGRAPHY A 1448.MAY(2016):121-126. |
入库方式： OAI收割
来源：过程工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。