Scale-up laccase production from Trametes versicolor stimulated by vanillic acid
文献类型：期刊论文
| 作者 | Wang, Ke-Feng1,2; Hu, Jian-Hua1,2; Guo, Chen1,2; Liu, Chun-Zhao1 |
| 刊名 | BIOPROCESS AND BIOSYSTEMS ENGINEERING
 |
| 出版日期 | 2016-07-01 |
| 卷号 | 39期号:7页码:1041-1049 |
| 关键词 | Trametes versicolor Vanillic acid Scale-up culture Laccase isoenzymes lcc |
| ISSN号 | 1615-7591 |
| 英文摘要 | An efficient strategy for laccase production in Trametes versicolor cultures was developed using vanillic acid as the inducer. The optimized vanillic acid treatment strategy consisted of exposing 2-day-old mycelia cultures to 80 mg/L vanillic acid. After 4 days, laccase activity of 588.84 U/L was achieved in flasks which represented a 1.79-fold increase compared to the control. In 200-L airlift bioreactor, the maximal laccase activity reached up to 785.12 U/L using the optimized vanillic acid treatment strategy. The zymograms of culture supernatants revealed three bands with laccase activity, among which Lac1 and Lac2 were abundant laccase isoforms constitutively expressed, and Lac3 was an inducible isozyme by vanillic acid. The results of real-time quantitative PCR showed that the transcription level of lcc in T. versicolor cultures grown with vanillic acid for 7 days was about 5.64-fold greater than that without vanillic acid in flasks. In 200-L airlift bioreactor cultures of T. versicolor with addition of vanillic acid, the transcript level of lcc at day 7 was 2.62-fold higher than that in flasks with vanillic acid due to the good mass transfer and oxygen supply in the bioreactor system. This study provides a basis for understanding the induction mechanism of vanillic acid for laccase production and has good potential for industrial applications. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Engineering, Chemical |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Engineering |
| 关键词[WOS] | PLEUROTUS-SAJOR-CAJU ; GENE-EXPRESSION ; AROMATIC-COMPOUNDS ; DIFFERENTIAL REGULATION ; SUBMERGED CULTURES ; FUNGI ; DECOLORIZATION ; OPTIMIZATION ; INDUCTION ; DYES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000378566700003 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/21178]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Ke-Feng,Hu, Jian-Hua,Guo, Chen,et al. Scale-up laccase production from Trametes versicolor stimulated by vanillic acid[J]. BIOPROCESS AND BIOSYSTEMS ENGINEERING,2016,39(7):1041-1049. |
| APA | Wang, Ke-Feng,Hu, Jian-Hua,Guo, Chen,&Liu, Chun-Zhao.(2016).Scale-up laccase production from Trametes versicolor stimulated by vanillic acid.BIOPROCESS AND BIOSYSTEMS ENGINEERING,39(7),1041-1049. |
| MLA | Wang, Ke-Feng,et al."Scale-up laccase production from Trametes versicolor stimulated by vanillic acid".BIOPROCESS AND BIOSYSTEMS ENGINEERING 39.7(2016):1041-1049. |
入库方式： OAI收割
来源：过程工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。