Novel ultraviolet (UV)-curable enzymatic antibacterial coatings were designed and fabricated. The coatings were prepared by simply mixture of castor oil-based cationic waterborne polyurethane-acrylate dispersion (WPUA) and acryloyl chloride modified lysozyme followed by UV curing. The covalent incorporation of lysozyme into the coatings was proved by enzyme leakage and IR analysis of the coatings. As the lysozyme content increased from 0 to 2.5% (w/w), the tensile strength of the coating enhanced by 37% (1.1 MPa vs 0.8 MPa), and the contact angle increased by 10% (73.1 degrees vs 80.6 degrees). Those changes are possibly due to enhancement of crosslinking degree and hydrogen. bonding induced by modified lysozyme. Based on live/dead staining and colonies number assay, the coatings containing lysozyme (2.5%, w/w) were highly efficient in killing Staphylococcus aureus (91.3%) and Escherichia coli (62.9%) via degradation of cell wall within 2 h, and the colonies number for both strains was significantly decreased to less than 1% after intubation for 24h. In addition, the killing efficiency of the coatings remained 68.3% after six continuous uses. Such UV-curable waterborne polyurethane coatings containing lysozyme (UWPU-L) may be useful in anti-bacterial stains on various common surfaces in hospital settings and food packaging. (C) 2016 Elsevier B.V. All rights reserved.