Enzymatic Hydrolysis of Alginate to Produce Oligosaccharides by a New Purified Endo-Type Alginate Lyase
文献类型：期刊论文
| 作者 | Zhu, Benwei1,2; Chen, Meijuan1; Yin, Heng3; Du, Yuguang4; Ning, Limin1 |
| 刊名 | MARINE DRUGS
 |
| 出版日期 | 2016-06-01 |
| 卷号 | 14期号:6页码:108 |
| 关键词 | alginate lyase Cellulophaga enzymatic hydrolysis oligosaccharides |
| ISSN号 | 1660-3397 |
| 英文摘要 | Enzymatic hydrolysis of sodium alginate to produce alginate oligosaccharides has drawn increasing attention due to its advantages of containing a wild reaction condition, excellent gel properties and specific products easy for purification. However, the efficient commercial enzyme tools are rarely available. A new alginate lyase with high activity (24,038 U/mg) has been purified from a newly isolated marine strain, Cellulophaga sp. NJ-1. The enzyme was most active at 50 degrees C and pH 8.0 and maintained stability at a broad pH range (6.0-10.0) and temperature below 40 degrees C. It had broad substrate specificity toward sodium alginate, heteropolymeric MG blocks (polyMG), homopolymeric M blocks (polyM) and homopolymeric G blocks (polyG), and possessed higher affinity toward polyG (15.63 mM) as well as polyMG (23.90 mM) than polyM (53.61 mM) and sodium alginate (27.21 mM). The TLC and MS spectroscopy analysis of degradation products suggested that it completely hydrolyzed sodium alginate into oligosaccharides of low degrees of polymerization (DPs). The excellent properties would make it a promising tool for full use of sodium alginate to produce oligosaccharides. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Chemistry, Medicinal |
| 研究领域[WOS] | Pharmacology & Pharmacy |
| 关键词[WOS] | OLIGOALGINATE LYASE ; CYTOKINE PRODUCTION ; DEGRADING ENZYMES ; PURIFICATION ; MACROPHAGES ; EXPRESSION ; INDUCTION ; CLONING ; CELLS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000378770300005 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/21179]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Nanjing Univ Chinese Med, Preclin Med Coll, Nanjing 210023, Jiangsu, Peoples R China 2.Nanjing Univ Technol, Coll Food Sci & Light Ind, Nanjing 211816, Jiangsu, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 4.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Benwei,Chen, Meijuan,Yin, Heng,et al. Enzymatic Hydrolysis of Alginate to Produce Oligosaccharides by a New Purified Endo-Type Alginate Lyase[J]. MARINE DRUGS,2016,14(6):108. |
| APA | Zhu, Benwei,Chen, Meijuan,Yin, Heng,Du, Yuguang,&Ning, Limin.(2016).Enzymatic Hydrolysis of Alginate to Produce Oligosaccharides by a New Purified Endo-Type Alginate Lyase.MARINE DRUGS,14(6),108. |
| MLA | Zhu, Benwei,et al."Enzymatic Hydrolysis of Alginate to Produce Oligosaccharides by a New Purified Endo-Type Alginate Lyase".MARINE DRUGS 14.6(2016):108. |
入库方式： OAI收割
来源：过程工程研究所
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