High-titer-ethanol production from cellulosic hydrolysate by an engineered strain of Saccharomyces cerevisiae during an in situ removal process reducing the inhibition of ethanol on xylose metabolism
文献类型：期刊论文
| 作者 | Zhang, Bo1,4; Sun, Hongbing1,2; Li, Jing3; Wan, Yinhua3; Li, Yin1; Zhang, Yanping1 |
| 刊名 | PROCESS BIOCHEMISTRY
 |
| 出版日期 | 2016-08-01 |
| 卷号 | 51期号:8页码:967-972 |
| 关键词 | Cellulosic ethanol Ethanol inhibition Fermentation-pervaporation Saccharomyces cerevisiae Xylose catabolism |
| ISSN号 | 1359-5113 |
| 英文摘要 | Efficient xylose utilization is critical for the production of fuels from biomass hydrolysates. It is known that xylose catabolism is inhibited by glucose. In this study, we showed that ethanol also inhibits xylose catabolism. By introducing a xylose metabolic pathway into Saccharomyces cerevisiae and using evolutionary engineering, an engineered S. cerevisiae strain, W32N55, was obtained that can anaerobically ferment xylose to ethanol. The effect of ethanol on xylose utilization was investigated. The results showed that xylose catabolism was inhibited upon the addition of ethanol, and it resumed once ethanol was removed. Based on these results, a fermentation-pervaporation coupling process was developed. After the in situ removal of ethanol, 150 g/L glucose and 31 g/L xylose were consumed in 72 h, providing a total of 76 g/L ethanol and an overall total sugar yield of 0.42 g/g. We believe that this strain will be valuable to the bio-ethanol industry. (C) 2016 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering |
| 关键词[WOS] | FERMENTATION ; GLUCOSE ; PERVAPORATION ; EXPRESSION ; GROWTH ; XYL1 ; XKS1 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000380972500005 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/21285]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Microbial Physiol & Metab Engn, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 3.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China 4.China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Bo,Sun, Hongbing,Li, Jing,et al. High-titer-ethanol production from cellulosic hydrolysate by an engineered strain of Saccharomyces cerevisiae during an in situ removal process reducing the inhibition of ethanol on xylose metabolism[J]. PROCESS BIOCHEMISTRY,2016,51(8):967-972. |
| APA | Zhang, Bo,Sun, Hongbing,Li, Jing,Wan, Yinhua,Li, Yin,&Zhang, Yanping.(2016).High-titer-ethanol production from cellulosic hydrolysate by an engineered strain of Saccharomyces cerevisiae during an in situ removal process reducing the inhibition of ethanol on xylose metabolism.PROCESS BIOCHEMISTRY,51(8),967-972. |
| MLA | Zhang, Bo,et al."High-titer-ethanol production from cellulosic hydrolysate by an engineered strain of Saccharomyces cerevisiae during an in situ removal process reducing the inhibition of ethanol on xylose metabolism".PROCESS BIOCHEMISTRY 51.8(2016):967-972. |
入库方式： OAI收割
来源：过程工程研究所
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