Changes in tension regulates proliferation and migration of fibroblasts by remodeling expression of ECM proteins
文献类型：期刊论文
| 作者 | Jiang MM; Qiu JH; Zhang LL; Lu DY; Long M(龙勉) ; Che, L; Luo XD
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| 刊名 | EXPERIMENTAL AND THERAPEUTIC MEDICINE
 |
| 出版日期 | 2016 |
| 卷号 | 12期号:3页码:1542-1550 |
| 通讯作者邮箱 | lichen201501@sian.com ; xiangdongluo2015@sina.com |
| 关键词 | human skin fibroblast keratinocyte mechanical stretch extracellular matrix integrin calcium calmodulin-dependent serine protein kinase |
| ISSN号 | 1792-0981 |
| 产权排序 | [Jiang, Minmin; Qiu, Juhui; Zhang, Lingling; Chen, Li; Luo, Xiangdong] Third Mil Med Univ, Burn Res Inst, Southwest Hosp, 30 Gaotanyan St, Chongqing 400038, Peoples R China; [Jiang, Minmin] Peoples Hosp Guizhou Prov, Cent Lab, Guiyang 550002, Guizhou, Peoples R China; [Qiu, Juhui; Zhang, Lingling; Chen, Li; Luo, Xiangdong] Chinese Acad Sci, Inst Mech, Natl Key Lab Trauma & Burns & Chongqing Key Lab D, Beijing 100190, Peoples R China; [Lu, Dongyuan; Long, Mian] Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Natl Micrograv Lab, Beijing 100190, Peoples R China; [Lu, Dongyuan; Long, Mian] Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Beijing 100190, Peoples R China; [Chen, Li] Third Mil Med Univ, Southwest Hosp, Breast Dis Ctr, 30 Gaotanyan St, Chongqing 400038, Peoples R China |
| 通讯作者 | Luo, XD (reprint author), Third Mil Med Univ, Burn Res Inst, Southwest Hosp, 30 Gaotanyan St, Chongqing 400038, Peoples R China. ; Chen, L (reprint author), Third Mil Med Univ, Southwest Hosp, Breast Dis Ctr, 30 Gaotanyan St, Chongqing 400038, Peoples R China. |
| 中文摘要 | Wound healing is a complicated but highly organized process in which cell migration and proliferation are actively involved. However, the process by which mechanical stretch regulates the proliferation and migration of human skin fibroblasts (HFs) and keratinocytes is poorly understood. Using a house built mechanical stretch device, we examined the HFs extracellular matrix (ECM) components changes under non-stretch, static stretch or cyclic stretch conditions. We further investigated the changes in ECM component protein expression levels in keratinocytes and analyzed the effects of individual ECM component on keratinocyte proliferation and migration. Particularly, the roles of calcium/calmodulin-dependent serine protein kinase (CASK) in the HF proliferation under cyclic stretch were investigated. Cyclic stretch suppressed HF proliferation compared with HFs without stretch or with static stretch. Cyclic stretch also led to a significant reduction in the levels of collagen I and a marked increase of fibronectin in HFs ECM. By contrast, collagen I levels increased and fibronectin levels decreased in response to non-stretch and static stretch conditions. After cyclic stretch, the proliferation of keratinocytes was inhibited by the cyclic stretch-induced ECM in HFs. The inoculation of keratinocytes with single ECM component suggested that collagen I was more capable of inducing cell proliferation than fibronectin, while it had less impact on cell migration compared with fibronectin. Furthermore, cyclic stretch induced by proliferation inhibition was associated with altered integrin 1-CASK signal pathway. The present results demonstrated the existence of HF-ECM-keratinocyte cross-talk' in cutaneous tissues. Thus, the integrin 1-CASK signal pathway in HFs may be involved in the outside-in signal transduction of extracellular stretch and the altered ECM component expression. |
| 分类号 | Q4 |
| 类目[WOS] | Medicine, Research & Experimental |
| 研究领域[WOS] | Research & Experimental Medicine |
| 关键词[WOS] | human skin fibroblast ; keratinocyte ; mechanical stretch ; extracellular matrix ; integrin ; calcium ; calmodulin-dependent serine protein kinase |
| 收录类别 | SCI |
| 原文出处 | http://dx.doi.org/10.3892/etm.2016.3497 |
| 语种 | 英语 |
| WOS记录号 | WOS:000382446000047 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/59743]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 推荐引用方式 GB/T 7714 | Jiang MM,Qiu JH,Zhang LL,et al. Changes in tension regulates proliferation and migration of fibroblasts by remodeling expression of ECM proteins[J]. EXPERIMENTAL AND THERAPEUTIC MEDICINE,2016,12(3):1542-1550. |
| APA | Jiang MM.,Qiu JH.,Zhang LL.,Lu DY.,龙勉.,...&Luo XD.(2016).Changes in tension regulates proliferation and migration of fibroblasts by remodeling expression of ECM proteins.EXPERIMENTAL AND THERAPEUTIC MEDICINE,12(3),1542-1550. |
| MLA | Jiang MM,et al."Changes in tension regulates proliferation and migration of fibroblasts by remodeling expression of ECM proteins".EXPERIMENTAL AND THERAPEUTIC MEDICINE 12.3(2016):1542-1550. |
入库方式： OAI收割
来源：力学研究所
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