Combined cell surface carbonic anhydrase 9 and CD147 antigens enable high-efficiency capture of circulating tumor cells in clear cell renal cell carcinoma patients
文献类型：期刊论文
| 作者 | Liu, Shijie1,2,3; Tian, Zuhong2,3; Zhang, Lei4; Hou, Shuang5; Hu, Sijun2,3; Wu, Junshen1; Jing, Yuming1; Sun, Huimin1; Yu, Fei1; Zhao, Libo6 |
| 刊名 | ONCOTARGET
 |
| 出版日期 | 2016-09-13 |
| 卷号 | 7期号:37页码:59877-59891 |
| 关键词 | carbonic anhydrase 9 CD147 circulating tumor cells renal cell carcinoma |
| 英文摘要 | Circulating tumor cells (CTCs) have emerged as promising tools for noninvasive cancer detection and prognosis. Most conventional approaches for capturing CTCs use an EpCAM-based enrichment strategy, which does not work well in cancers that show low or no expression of EpCAM, such as renal cell carcinoma (RCC). In this study, we developed a new set of cell surface markers including CA9 and CD147 as alternative CTC-capture antigens specifically designed for RCC patients. We showed that the expression of both CA9 and CD147 was prevalent in a RCC patient cohort (n= 70) by immunohistochemical analysis, with both molecules in combination covering 97.1% of cases. The NanoVelcro platform combined with CA9-/CD147-capture antibodies demonstrated significantly higher efficiency for capturing both CTC-mimicking renal cancer cells and RCC CTCs in peripheral blood, compared to the conventional EpCAM-based method. Using immunofluorescence cytological validation at the single-cell level, we were able to identify bona fide CTCs in RCC patient blood following the well-accepted criteria in our CTC-capture system. We further demonstrated a significant association of CTC numbers as well as the CTC expression status of Vimentin, a mesenchymal marker, with disease progression, including pathologic features and clinical staging. These results provide new insights into developing novel, effective targets/approaches for capturing CTCs, making CTCs a valuable tool for improved cancer detection, prognosis and treatment in RCC. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/35097]  |
| 专题 | 化学研究所_分子纳米结构与纳米技术实验室 |
| 作者单位 | 1.Fourth Mil Med Univ, Xijing Hosp, Dept Urol, Xian 710032, Shaanxi, Peoples R China 2.Fourth Mil Med Univ, State Key Lab Canc Biol, Xian 710032, Shaanxi, Peoples R China 3.Fourth Mil Med Univ, Xijing Hosp Digest Dis, Xian 710032, Shaanxi, Peoples R China 4.Fourth Mil Med Univ, Dept Epidemiol, Xian 710032, Shaanxi, Peoples R China 5.Univ Calif Los Angeles, Calif Nanosyst Inst, Crump Inst Mol Imaging, Dept Mol & Med Pharmacol, Los Angeles, CA 90095 USA 6.Chinese Acad Sci, Inst Chem, Key Lab Mol Nanostruct & Nanotechnol, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China 7.Cedars Sinai Med Ctr, Samuel Oschin Comprehens Canc Inst, Dept Med, Urooncol Res Program, Los Angeles, CA 90048 USA 8.Natl Ctr Nanosci & Nanotechnol, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China 9.Washington State Univ, Coll Pharm, Dept Pharmaceut Sci, Spokane, WA 99202 USA |
| 推荐引用方式 GB/T 7714 | Liu, Shijie,Tian, Zuhong,Zhang, Lei,et al. Combined cell surface carbonic anhydrase 9 and CD147 antigens enable high-efficiency capture of circulating tumor cells in clear cell renal cell carcinoma patients[J]. ONCOTARGET,2016,7(37):59877-59891. |
| APA | Liu, Shijie.,Tian, Zuhong.,Zhang, Lei.,Hou, Shuang.,Hu, Sijun.,...&Shao, Chen.(2016).Combined cell surface carbonic anhydrase 9 and CD147 antigens enable high-efficiency capture of circulating tumor cells in clear cell renal cell carcinoma patients.ONCOTARGET,7(37),59877-59891. |
| MLA | Liu, Shijie,et al."Combined cell surface carbonic anhydrase 9 and CD147 antigens enable high-efficiency capture of circulating tumor cells in clear cell renal cell carcinoma patients".ONCOTARGET 7.37(2016):59877-59891. |
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来源：化学研究所
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