A Lysosome-Targeting Fluorescence Off-On Probe for Imaging of Nitroreductase and Hypoxia in Live Cells
文献类型：期刊论文
| 作者 | Zhou, Jin; Shi, Wen; Li, Li-Hong; Gong, Qiu-Yu; Wu, Xiao-Feng; Li, Xiao-Hua; Ma, Hui-Min |
| 刊名 | CHEMISTRY-AN ASIAN JOURNAL
 |
| 出版日期 | 2016-10-01 |
| 卷号 | 11期号:19页码:2719-2724 |
| 关键词 | cell hypoxia fluorescence imaging fluorescent probes imaging agents lysosome nitroreductase |
| 英文摘要 | A lysosome-targeting fluorescent off-on probe has been developed by one-step synthesis for detecting lysosomal nitroreductase and hypoxia. The probe is constructed by incorporating morpholine (a lysosome-targeting unit) into 4-nitro-1,8-naphthalimide (as a fluorochrome and specific substrate for nitroreductase), and the detection mechanism is based on the nitroreductase-catalyzed reduction of the probe to 4-amino-1,8-naphthalimide, accompanied by a large fluorescence enhancement at a wavelength of 543 nm. The probe shows an accurate lysosome-targeting ability with high selectivity and sensitivity to nitroreductase (detection limit: 2.2 ng mL(-1)). Notably, the probe has been used to image the change of lysosomal nitroreductase in live cells during hypoxia, revealing that the increase of nitroreductase in lysosomes may be smaller than that in the cytoplasm. In addition, the probe is expected to be useful for studying the function of nitroreductase in the acidic organelle of lysosomes. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/35109]  |
| 专题 | 化学研究所_活体分析化学实验室 |
| 作者单位 | Chinese Acad Sci, Beijing Natl Lab Mol Sci, Key Lab Analyt Chem Living Biosyst, Inst Chem, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Jin,Shi, Wen,Li, Li-Hong,et al. A Lysosome-Targeting Fluorescence Off-On Probe for Imaging of Nitroreductase and Hypoxia in Live Cells[J]. CHEMISTRY-AN ASIAN JOURNAL,2016,11(19):2719-2724. |
| APA | Zhou, Jin.,Shi, Wen.,Li, Li-Hong.,Gong, Qiu-Yu.,Wu, Xiao-Feng.,...&Ma, Hui-Min.(2016).A Lysosome-Targeting Fluorescence Off-On Probe for Imaging of Nitroreductase and Hypoxia in Live Cells.CHEMISTRY-AN ASIAN JOURNAL,11(19),2719-2724. |
| MLA | Zhou, Jin,et al."A Lysosome-Targeting Fluorescence Off-On Probe for Imaging of Nitroreductase and Hypoxia in Live Cells".CHEMISTRY-AN ASIAN JOURNAL 11.19(2016):2719-2724. |
入库方式： OAI收割
来源：化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。