Fluorogenic Enhancement of an in Vitro-Selected Peptide Ligand by Replacement of a Fluorescent Group
文献类型：期刊论文
| 作者 | Wang, Wei1,2; Zhu, Liping2; Hirano, Yoshinori3; Karirninavargani, Marziyeh2,4; Tada, Seiichi6; Zhang, Guanxin5; Uzawa, Takanori2,6; Zhang, Deqing5; Hirose, Takuji4; Taiji, Makoto3 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2016-08-16 |
| 卷号 | 88期号:16页码:7991-7997 |
| 英文摘要 | To prepare a fluorogenic peptide ligand which 'binds to an arbitrary target, we previously succeeded in seeking a fluorogenic ligand to calmodulin using in vitro selection. In this study the environment-sensitive fluorescent group in the selected peptide ligand was replaced with other fluorescent groups to ad, the possibility-to increase the fluorogenic activity. Surface plasmon resonance measurement exhibited that the binding affinity was held even after the replacement. However, the replacement significantly affected the fluorogenic activity. It depended on the kind of incorporated fluorophors and linker length. As a result, the incorporation of 4-N,N-dimethylamino-1,8-naphthalimide enhanced the fluorescence intensity over 100-fold' in the presence of target calcium-bound calModulin. This study demonstrated that the functionality of in vitro selected peptide can, be tuned with keeping the binding affinity. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/35346]  |
| 专题 | 化学研究所_有机固体实验室 |
| 作者单位 | 1.Chinese Acad Sci, High Field Magnet Lab, Hefei 230031, Anhui, Peoples R China 2.RIKEN, Nano Med Engn Lab, 2-1 Hirosawa, Wako, Saitama 3510198, Japan 3.RIKEN, Quantitat Biol Ctr, Computat Biol Res Core, Lab Computat Mol Design, 2F,QBiC Bldg B,6-2-4 Furuedai, Suita, Osaka 5650874, Japan 4.Saitama Univ, Grad Sch Sci & Engn, Sakura Ku, 255 Shimo Okubo, Saitama 3388570, Japan 5.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Key Lab Organ Solids, Beijing 100190, Peoples R China 6.RIKEN, Ctr Emergent Matter Sci, Emergent Bioengn Mat Res Team, 2-1 Hirosawa, Wako, Saitama 3510198, Japan |
| 推荐引用方式 GB/T 7714 | Wang, Wei,Zhu, Liping,Hirano, Yoshinori,et al. Fluorogenic Enhancement of an in Vitro-Selected Peptide Ligand by Replacement of a Fluorescent Group[J]. ANALYTICAL CHEMISTRY,2016,88(16):7991-7997. |
| APA | Wang, Wei.,Zhu, Liping.,Hirano, Yoshinori.,Karirninavargani, Marziyeh.,Tada, Seiichi.,...&Ito, Yoshihiro.(2016).Fluorogenic Enhancement of an in Vitro-Selected Peptide Ligand by Replacement of a Fluorescent Group.ANALYTICAL CHEMISTRY,88(16),7991-7997. |
| MLA | Wang, Wei,et al."Fluorogenic Enhancement of an in Vitro-Selected Peptide Ligand by Replacement of a Fluorescent Group".ANALYTICAL CHEMISTRY 88.16(2016):7991-7997. |
入库方式： OAI收割
来源：化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。