绿荧光蛋白的双光子激发的荧光特性研究
文献类型：期刊论文
| 作者 | 夏安东; 蒋诗平; 朱纪军; 沈耀春; 陆祖宏; 黄文浩 |
| 刊名 | 光谱学与光谱分析
 |
| 出版日期 | 2001 |
| 卷号 | 21期号:4页码:435 |
| 通讯作者 | Xia, AD (reprint author), SE Univ Nanjing, Natl Lab Mol & Biomol Elect, Nanjing 210096, Peoples R China. |
| 中文摘要 | 利用双光子激发方式研究了重组绿荧光蛋白 (recombinantgreenfluorescentprotein,简称rGFP)的光转换特性 ,研究结果表明rGFP具有较强的双光子激发荧光。双光子激发的荧光偏振光谱表明rGFP在辐照前质子态和去质子态之间存在着有效的能量转移过程。rGFP辐照后导致生色团构象的变化 ,部分阻断了rGFP内源的氨基酸与生色团之间的能量转移过程 ,导致rGFP的双光子激发的荧光强度下降。观察到rGFP的三光子激发的荧光特性 ,这种三光子激发的荧光主要来源于rGFP内源的氨基酸 ( |
| 英文摘要 | The photoconversion process of recombinant green fluorescent protein (rGFP) was investigated by two-photon. excitation. The results indicated that the rGFP had very strong two-photon excitation fluorescence. The changes of two-photon- induced fluorescence polarization suggest that there is a proton transfer process between two different proton states of rGFP chromophores. The conformation of rGFP chromophores could be changed upon illumination, which partly block the energy transfer processes from amino acid residues to chromophores in rGFP, and result in the decrease of two-photoninduced fluorescence intensity. The fluorescence from amino acid residues in rGFP was also observed by three-photon excitation., which resulted from the blocked amino acid residues in rGFP. These results suggested that it is necessary to optimize rGFP excitation and detection for quantitative fluorescence microscopy. |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000170980700006 |
| 版本 | 出版稿 |
| 源URL | [http://ir.siom.ac.cn/handle/181231/17353]  |
| 专题 | 上海光学精密机械研究所_强场激光物理国家重点实验室 |
| 作者单位 | 1.SE Univ Nanjing, Natl Lab Mol & Biomol Elect, Nanjing 210096, Peoples R China 2.Univ Sci & Technol China, Dept Precis Machinery & Instrumentat, Hefei 230026, Peoples R China 3.Univ Sci & Technol China, Natl Synchrotron Radiat Lab, Hefei 230029, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Opt & Fine Mech, Shanghai 201800, Peoples R China |
| 推荐引用方式 GB/T 7714 | 夏安东,蒋诗平,朱纪军,等. 绿荧光蛋白的双光子激发的荧光特性研究[J]. 光谱学与光谱分析,2001,21(4):435. |
| APA | 夏安东,蒋诗平,朱纪军,沈耀春,陆祖宏,&黄文浩.(2001).绿荧光蛋白的双光子激发的荧光特性研究.光谱学与光谱分析,21(4),435. |
| MLA | 夏安东,et al."绿荧光蛋白的双光子激发的荧光特性研究".光谱学与光谱分析 21.4(2001):435. |
入库方式： OAI收割
来源：上海光学精密机械研究所
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