Single-band upconversion nanoprobes for multiplexed simultaneous in situ molecular mapping of cancer biomarkers
文献类型：期刊论文
| 作者 | Zhou, Lei; Wang, Rui; Yao, Chi; Li, Xiaomin; Wang, Chengli; Zhang, Xiaoyan; Xu, Congjian; Zeng, Aijun; Zhao, Dongyuan; Zhang, Fan |
| 刊名 | nat. commun.
 |
| 出版日期 | 2015 |
| 卷号 | 6页码:6938 |
| 通讯作者 | zhang, f (reprint author), fudan univ, state key lab mol engn polymers, dept chem, ichem collaborat innovat ctr chem energy mat, shanghai 200433, peoples r china. |
| 英文摘要 | the identification of potential diagnostic markers and target molecules among the plethora of tumour oncoproteins for cancer diagnosis requires facile technology that is capable of quantitatively analysing multiple biomarkers in tumour cells and tissues. diagnostic and prognostic classifications of human tumours are currently based on the western blotting and single-colour immunohistochemical methods that are not suitable for multiplexed detection. herein, we report a general and novel method to prepare single-band upconversion nanoparticles with different colours. the expression levels of three biomarkers in breast cancer cells were determined using single-band upconversion nanoparticles, western blotting and immunohistochemical technologies with excellent correlation. significantly, the application of antibody-conjugated single-band upconversion nanoparticle molecular profiling technology can achieve the multiplexed simultaneous in situ biodetection of biomarkers in breast cancer cells and tissue specimens and produce more accurate results for the simultaneous quantification of proteins present at low levels compared with classical immunohistochemical technology. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 版本 | 出版稿 |
| 源URL | [http://ir.siom.ac.cn/handle/181231/14201]  |
| 专题 | 上海光学精密机械研究所_信息光学与光电技术实验室 |
| 作者单位 | 1.[Zhou, Lei 2.Wang, Rui 3.Yao, Chi 4.Li, Xiaomin 5.Wang, Chengli 6.Zhao, Dongyuan 7.Zhang, Fan] Fudan Univ, State Key Lab Mol Engn Polymers, Dept Chem, IChEm Collaborat Innovat Ctr Chem Energy Mat, Shanghai 200433, Peoples R China 8.[Zhang, Xiaoyan 9.Xu, Congjian] Fudan Univ, Obstet & Gynecol Hosp, Shanghai 200011, Peoples R China 10.[Zeng, Aijun] Chinese Acad Sci, Shanghai Inst Opt & Fine Mech, Shanghai 201800, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Lei,Wang, Rui,Yao, Chi,et al. Single-band upconversion nanoprobes for multiplexed simultaneous in situ molecular mapping of cancer biomarkers[J]. nat. commun.,2015,6:6938. |
| APA | Zhou, Lei.,Wang, Rui.,Yao, Chi.,Li, Xiaomin.,Wang, Chengli.,...&Zhang, Fan.(2015).Single-band upconversion nanoprobes for multiplexed simultaneous in situ molecular mapping of cancer biomarkers.nat. commun.,6,6938. |
| MLA | Zhou, Lei,et al."Single-band upconversion nanoprobes for multiplexed simultaneous in situ molecular mapping of cancer biomarkers".nat. commun. 6(2015):6938. |
入库方式： OAI收割
来源：上海光学精密机械研究所
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