一种高性能金标条阅读仪的研制
文献类型：期刊论文
| 作者 | 陈贝特; 黄立华; 郭凯; 黄惠杰 |
| 刊名 | 中国激光
 |
| 出版日期 | 2013 |
| 卷号 | 40期号:7页码:704001 |
| 其他题名 | Development of a High Performance Reader for Colloidal Gold Lateral Flow Strip |
| 通讯作者 | 陈贝特, 中国科学院上海光学精密机械研究所信息光学与光电技术实验室, 上海 201800, 中国. ; 黄立华, 中国科学院上海光学精密机械研究所信息光学与光电技术实验室, 上海 201800, 中国. ; 郭凯, 中国科学院上海光学精密机械研究所信息光学与光电技术实验室, 上海 201800, 中国. ; 黄惠杰, 中国科学院上海光学精密机械研究所信息光学与光电技术实验室, 上海 201800, 中国. |
| 中文摘要 | 为使金标免疫层析技术能对目标被检物进行高灵敏度快速定量检测,研制了一种基于CMOS图像传感器的金标条阅读仪。根据纳米金颗粒对绿光的强烈吸收特性,使用绿光LED为照明光源;依据空白金标试纸条图像的灰度分布调节LED光强和位置,使成像区域的照明均匀性达到最佳;依据自定义评价参数调节CMOS图像传感器的成像控制参数,使图像质量达到最佳;使用空白金标试纸条图像数据做背景校正,再通过特定算法计算出样品中目标被检物的浓度。使用该金标条阅读仪对滴加有心肌肌钙蛋白I(cTnI)标准样品的金标试纸条进行检测,在质量浓度为0.25~64 ng/mL范围内具有良好的4-PL响应特性,相关系数R~2>0.99;使用该金标条阅读仪对上述浓度范围的金标试纸条各进行20次重复测量,其中,对高浓度金标试纸条(64 ng/mL)测量结果的变异系数为0.134%,对低浓度金标试纸条(0.25 ng/mL)测量结果的变异系数为2.790%;功能灵敏度优于0.25 ng/mL。该金标条阅读仪具有重复性好、灵敏、快速、低功耗、小型化等特点。 |
| 英文摘要 | A colloidal gold lateral flow (LF) strip reader based on CMOS image sensor, characterized by ultrasensitive and high repeatable, is developed. As gold nano-particles absorb green light strongly, green LEDs are used as the light source in the reader. In order to get the best illumination uniformity, gray value distribution of blank strip image is used to adjust the location and intensity of LEDs. CMOS control parameters are specified to get the most suitable image quality for image analyzing by self-defining parameters. Blank strip is used to calibrate the background signal. The quantitative result is derived from the ratio of integral reflection optical density of T line signal to C line signal. LF strips of cardiac troponin I (cTnI) with standard mass concentration gradients from 0.25 ng/ml to 64 ng/mL are detected. The 4-PL quantitative model is used to fitting standard working curve, whose correlation of coefficient (R~2) is more than 0.99. The coefficient of variation (CV) of 20 times repetitive experiments of 0.25 ng/mL is 2.790%, and 64 ng/mL is 0.134%. The functional sensitivity (FS) is better than 0.25 ng/mL. The reader is sensitive, stable, simple, and suitable for field detection. |
| 收录类别 | EI |
| 语种 | 中文 |
| 版本 | 出版稿 |
| 源URL | [http://ir.siom.ac.cn/handle/181231/14902]  |
| 专题 | 上海光学精密机械研究所_信息光学与光电技术实验室 |
| 作者单位 | 1.Chen Beite, Laboratory of Information Optics and Opto-Electronic Technology, Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800, China. 2.Huang Lihua, Laboratory of Information Optics and Opto-Electronic Technology, Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800, China. 3.Guo Kai, Laboratory of Information Optics and Opto-Electronic Technology, Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800, China. 4.Huang Huijie, Laboratory of Information Optics and Opto-Electronic Technology, Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800, China. |
| 推荐引用方式 GB/T 7714 | 陈贝特,黄立华,郭凯,等. 一种高性能金标条阅读仪的研制[J]. 中国激光,2013,40(7):704001. |
| APA | 陈贝特,黄立华,郭凯,&黄惠杰.(2013).一种高性能金标条阅读仪的研制.中国激光,40(7),704001. |
| MLA | 陈贝特,et al."一种高性能金标条阅读仪的研制".中国激光 40.7(2013):704001. |
入库方式： OAI收割
来源：上海光学精密机械研究所
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