激光镊子拉曼光谱仪研制及其应用研究
文献类型：学位论文
| 作者 | 邓见辽 |
| 学位类别 | 博士 |
| 答辩日期 | 2005 |
| 授予单位 | 中国科学院上海光学精密机械研究所 |
| 导师 | 王育竹 |
| 关键词 | 光镊 拉曼光谱 血红细胞 直肠癌 |
| 其他题名 | Development and applications of Laser tweezers Raman spectroscopy |
| 中文摘要 | 本论文介绍了激光镊子拉曼光谱仪的原理、系统实现及其应用研究。第一章介绍了拉曼散射的历史、基本原理和拉曼光谱技术的发展及其在生物医学中的应用。第二章详细介绍了光镊的基本原理，激光镊子拉曼光谱仪各个部分的构造及系统的校准及性能评估。第三章介绍了用脉冲光镊俘获和操纵吸附在玻璃表面粒子的实验，然后提出我们的理论分析并进行数值模拟来解释他们的实验结果。我们的理论计算结果与实验结果定性相符。第四章介绍了激光镊子拉曼光谱仪在生物医学中的应用研究。它包括两部分：第一部分是利用拉曼光谱研究酒精对人体血红细胞的影响。血红细胞浸泡在20％酒精溶液25分钟出现玩2的明显减小可能与血红蛋白与酒精相互作用导致的血红细胞变性有关。当酒精浓度增加时玩2的减小变得越来越快越来越明显证实了这一推测。当酒精浓度超过30％时，血红细胞在小于3分钟的时间里破裂，细胞结构完全被破坏。另一部分是利用单细胞拉曼光谱技术识别直肠癌细胞。我们利用正常直肠细胞和直肠癌细胞拉曼光谱之间的差异，可以正确的预测20个癌细胞中的17个和20个正常细胞中的19个，这一分类的灵敏性和特异性分别为85％和95％。第五章是博士期间完成的测量极弱相干光在铆蒸汽盒中的透射率实验。 |
| 英文摘要 | The thesis presents the principle, development, and applications of laser tweezers Raman spectroscopy (LTRS). Chapter 1 introduces the history and basic principle of Raman effect, the technique development and applications in biomedicine of Raman spectroscopy. Chapter 2 presents the principle of laser tweezers, configuration and calibration of laser tweezers Raman spectroscopy. Chapter 3 first introduces the experiment named as optical levitation and manipulation of stuck particles with pulsed optical tweezers, then presents our theoretical analysis and the numerical modeling to explain their experimental results. The result of our numerical modeling is qualitatively consistent with the experimental result. Chapter 4 presents the applications of LTRS in biomedicine. It includes two parts. The first part concerns study of the effect of alcohol on single human red blood cells using NIR LTRS. The significant decrease of I752 over the period of 25 minutes after the RBCs are diluted in 20% alcohol solution is probably related to the degradation of the RBCs resulting from the hemoglobin-alcohol interaction. It is also verified by the fact that the decrease of I752 become more rapid and more prominent as the alcohol concentration increases. When the alcohol concentration goes higher than 30%, the RBCs break down within 3 minutes and the cell structures collapse. The second part concerns distinguishing rectum cancer cells using Raman spectroscopy at single cell level. We are able to correctly predict 17 of 20 cancer cells and 19 of 20 normal cells based on the spectra differences between the normal rectal cell and rectum cancer cell. Thus, the sensitivity and specificity of this classification is 85% and 95% respectively. Chapter 5 presents the experiment, done during studying for doctor degree, named as measuring transmittance in rubidium vapour cells using an extremely weak coherent field of light. |
| 语种 | 中文 |
| 源URL | [http://ir.siom.ac.cn/handle/181231/15531]  |
| 专题 | 上海光学精密机械研究所_学位论文 |
| 推荐引用方式 GB/T 7714 | 邓见辽. 激光镊子拉曼光谱仪研制及其应用研究[D]. 中国科学院上海光学精密机械研究所. 2005. |
入库方式： OAI收割
来源：上海光学精密机械研究所
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