超高分辨光学成像技术的研究
文献类型：学位论文
| 作者 | 刘力 |
| 学位类别 | 博士 |
| 答辩日期 | 2001 |
| 授予单位 | 中国科学院上海光学精密机械研究所 |
| 导师 | 徐至展 |
| 关键词 | 超分辨 差分共焦显微术、光瞳滤波器 共焦扫描成像系统 光存储系统 高散射介质 多光子荧光 |
| 中文摘要 | 九十年代以来，超高分辨光学显微成像技术获得了迅猛的发展。本论文阐述了光学超分辨研究的现状，并从多角度对分辨率问题进行了分析。着重开展了三个方面的研究工作。一，建立在差分共焦显微术基础上的超分辨技术研究。二，利用全局优化算法设计超分辨光瞳滤波器。三，进行了多光子荧光对高散射介质中的物体成像的实验研究。本论文的主要创新成果如下：第一，发展并改进了差分共焦显微技术，差分共焦显微术利用共焦扫描的纵向响应曲线，与微分插值处理相结合，可以获得横向亚微米、纵向纳米级的分辨，具有快速、直观、精确的特色。1．拓展了差分共焦显微术的应用范围。首次将其用于实时地、较大动态范围地自动控制监测压电陶瓷的逆压电伸缩特性，包括压电陶瓷的逆压电系数、线性度及迟滞性等。将光学系统纵向分辨率提高到纳米量级。2．首次给出了差分共焦显微术与环型光瞳滤波器相结合的技术路线，扩展了差分共焦显微镜的测量动态范围，并且提高了它的横向分辨率。使本文所发展的差分共焦显微术具有更大的应用范围。并且给出了光栅表面形貌的成像实例。第二，较全面的研究了利用光瞳滤波器改进特定系统光学分辨率的问题，论述了光瞳滤波器超分辨研究在共焦扫描成像系统和光存储系统中应用的可行性。创造性地利用约束全局优化算法CGO设计了多种不同功能的位相型光瞳滤波器。1．分别设计了适用于光存储系统和共焦系统的光瞳滤波器，可以改善系统横向分辨率。2．设计了改善系统轴向分辨率的光瞳滤波器，将其用于共焦扫描成像系统以提高系统的层析能力。3．设计了改善系统三维分辨率的光瞳滤波器，用一个该光瞳滤波器与共焦系统相结合时便能获得三维超分辨效果。利用共焦系统和光瞳滤波器相结合的方法来获取超高分辨率信息，使利用辐射光照明成像的经典光学显微术获得新生，同时也为光学显微术提出一条新途径。是目前显微技术原理和方法方面的再发展和突破。该选题具有重大的科学意义。第三，采用多光子荧光技术，利用皮秒红外激光作为光源，创建了新的侧接收成像方式，建立了聚焦多光子荧光扫描成像系统。利用该装置进行了高散射介质的穿透深度的实验研究。初步实验结果表明该系统能分辨埋藏在高散射介质中的标准针，穿透深度为2．5cm。该课题为探索在活性生物组织癌症的早期诊断等方面的应用提供了实验依据，对于实际应用和理论研究都非常重要。 |
| 英文摘要 | The optical super-resolution imaging technique is being developed rapidly. In this thesis, current research of optical super-resolution is presented, and the concept of the resolution hasbeen discussed thoroughly from many different ways. The research in this thesis includes three aspects. One is the development of the differential confocal microscopy (DCM), another is the design of the phase-only optical pupil filter, and the last is the experiment research of imaging technology for detecting objects hidden in highly scattering media with focusing fluorescence scanning system. First, DCM was developed. The technology offers great advantages in precision, simplicity, and measuremnent speed. 1. The use of DCM was generalized. For the first time, DCM is developed to be used for real-time and large dynamic range measuring the converse piezoelectric extension of the piezoelectric ceramics (PZT) actuator, including its converse piezoelectric constant, linearity and hysteresis. The depth resolution is up to several nanometers. 2.For the first time, DCM and an annular pupil filter were combined together. Experimental and academic results prove that this method can extend the axial dynamic range and improve the transverse resolution of three-dimension profilometry by sacrificing axial resolution. Experimentally, surface of a reflection grating was measured. Second, feasibility of using optical super-resolution pupil filter in both confocal imaging system and optical storage system is discussed. Designing super-resolution pupil filter with constrained global optimization algorithm. Design consideration and solution for application such as confocal imaging system and optical storage system. 1.Optical pupil filter was provided, which can improve transverse resolution of confocal imaging system or optical storage system. 2.Optical pupil filter used in confocal imaging system was provide, which can improve the axial resolution and section property. 3.Optical pupil filter was designed, which can achieve 3-D superresolution when it is applied to confocal imaging system. Last, a focusing fluorescence scanning system with multiphoton fluorescence technology is finished. Using the infrared light of 1.06 micrometer, imaging of a standard pin hidden in highly scattering media is achieved with collecting fluorescence in the direction vertical to the excitation light beam. The penetration depth is 2.5 micrometer. |
| 语种 | 中文 |
| 源URL | [http://ir.siom.ac.cn/handle/181231/15578]  |
| 专题 | 上海光学精密机械研究所_学位论文 |
| 推荐引用方式 GB/T 7714 | 刘力. 超高分辨光学成像技术的研究[D]. 中国科学院上海光学精密机械研究所. 2001. |
入库方式： OAI收割
来源：上海光学精密机械研究所
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