基于ARM 微控制器的上转换发光生物传感器的研制
文献类型：学位论文
| 作者 | 谢承科 |
| 学位类别 | 硕士 |
| 答辩日期 | 2009 |
| 授予单位 | 中国科学院上海光学精密机械研究所 |
| 导师 | 黄惠杰 |
| 关键词 | 光学生物传感器 上转换发光技术 ARM 微控制器 免疫层析信号处理 |
| 其他题名 | Development of ARM microcontroller based UPT Biosensor |
| 中文摘要 | 随着科学技术的飞速发展，人们的生活水平不断提高。然而，新的病毒不断出现；食品安全问题日益凸显；国际生物恐怖袭击愈演愈烈等等。这些问题时刻威胁着人们的健康生活，甚至影响社会的发展和稳定。因此，能够使用安全有效的方法实现对病毒、有害物质和生物恐怖剂等的快速及时检测成为迫切需要。上转换发光生物传感器（Up-converting Phosphor Technology based biosensor, UPT生物传感器）就是为了满足这种应用需求而产生的。 UPT生物传感器是一种以上转换发光材料（Up-Converting Phosphor，UCP）为标记物的新型免疫分析生物传感器。UCP是由稀土离子掺杂于某一晶体晶格中构成的亚微米或纳米级颗粒，具有在红外光激发下发射出可见光的独特性能。对UCP颗粒进行一系列的表面修饰与活化后，可将其作为生物标记物与多种生物活性分子相结合，应用于生物检测领域。运用这种技术可对被检物进行无损伤检测，并且具有检测灵敏度高、速度快、可进行多重检测、使用安全等众多优点。 本论文的主要内容如下： 1. 综述了上转换发光材料的组成、发光机制及其应用，重点介绍了UCP作为生物标记物在生物检测中的应用及其优点。 2. 探讨了荧光、延迟荧光和磷光的区别，并研究了本论文所用的UCP标记物材料NaYF4: Yb3+, Er3+的上转换发光特性；在此基础上，介绍了UCP标记物的制备及作为它的反应载体UPT免疫层析试纸条的研制。 3. 详细阐述了基于ARM微控制器的UPT生物传感器的硬件设计。该仪器器主要由光学系统、光电转换器件、试纸条扫描机构和控制系统组成。采用对试纸条上所结合的UCP标记物的上转换发光进行扫描检测的方式工作。重点讨论了控制系统的硬件设计，以ARM微控制器为核心，构建了以STM32F103芯片、信号采集电路、电机驱动模块、射频识别（Radio Frequency Identification，简称RFID）读写模块、键盘、液晶显示模块、存储器、SD存储卡、微型打印机为一体的UPT生物传感器的硬件平台。 4. 在详细阐述系统应用程序设计的基础上，提出了一种基于互相关原理的自适应功能带边界定位算法。应用程序的设计采用自底向上的方法，先完成各基本模块的程序设计，再实现总体功能。自适应功能带边界定位算法可以准确地确定检测带与质控带的位置，大大提高了仪器的智能化程度。 5. 为了考核UPT-4生物传感器的性能，以双抗原夹心模式UPT试纸条对系列浓度鼠疫F1抗体标准样品进行了检测。实验结果表明，该仪器的检测灵敏度达到ng/ml量级；同一试纸条在相同条件下的检测重复性（变异系数）CV=1.5%；同一试纸条在不同条件下的检测稳定性（变异系数）CV3.3%；在0~450ng/ml浓度范围内具有良好的线性响应特性，其线性相关系数R2=94.27%。 6. 总结了本论文取得的主要成果，列出了主要创新点，并提出了改进措施。 |
| 英文摘要 | With the rapid development of science and technology, people’s life is getting better and better. However, new viruses emerge continuously; food safety problem is becoming increasingly prominent; international biological terrorist attacks are more and more serious, and etc. These problems threaten people''s healthy life all the time, and even impact the development and stability of the society. Therefore, there is an urgent need that viruses, harmful substances, biothreat agents, and etc can be detected quickly and timely using a safe and effective method. In order to meet this application demand, an Up-converting Phosphor Technology based biosensor (UPT biosensor) has been developed. The UPT biosensor is a novel biosensor used in immunoassay, in which Up-Converting Phosphor (UCP) is used as the biological marker. UCP is submicron or nano-sized ceramic particles formed by doping rare earth ions into a crystal. It can emit visible phosphorescent light when excited by infrared radiation. After a series of surface modification and activation, it can be used as the biomarker in biological detection area by combining it with various kinds of biological active molecular. This technique can realize non-invasive detection with merit of rapidness, high sensitivity, stable performance, multi-analyte detection, safe operation, and etc. The primary contributions of this thesis are given in the following aspects: 1. A summary of the composition, light-emitting mechanism and practical application of the UCP are given. In particular, UCP is served as a biomarker in the biological detection area and it has many advantages. 2. The distinctions between fluorescence, delayed fluorescence and phosphorescence are investigated. The up-converting luminescence properties of the UCP——NaYF4: Yb3+, Er3+ used in this paper are described. The spherical and homogeneous UCP particles have been synthesized. At the same time, the UPT-based immune chromatographic test strip (test strip) has been developed, which is the reactor carrier for the biological active molecular marked with UCP biomarkers. 3. The hardware design of the ARM microcontroller based UPT biosensor is expatiated in detail. It includes an optical system, a photoelectronic device, a test strip scanning mechanics and a control system. The UCP biomarker distribution along the test strip is obtained via scanning approach. Focus on the hardware design of the control system, which is constructed by an ARM microcontroller STM32F103, signal acquisition circuits, a motor driver module, a RFID reader module, a keyboard, a LCD module, a memory, a SD memory card and a micro printer. 4. The development of the system application software is expatiated in detail. Further, an adaptive borderline locating algorithm is proposed. The system application software is developed by the bottom-up approach. The program of each basic module is completed firstly, and then the whole system is constructed. The adaptive borderline locating algorithm can fix the positions of the detection line and the control line accurately, which greatly improves the intelligence of the biosensor. 5. In order to evaluate the performance of the UPT-4 biosensor, double-antigen sandwich format test strips are used to detect plague F1 antibody standard samples with serial concentrations. The results show that the biosensor has high sensitivity (~ng/ml), high repeatability and stability (CV3.3%), excellent linear response performance (correlation coefficient R2=94.27%) within a concentration range from 0 to 450 ng/ml. 6. In the end, achievements of this thesis are summarized, innovative aspects are listed, and some further improvements are proposed. |
| 语种 | 中文 |
| 源URL | [http://ir.siom.ac.cn/handle/181231/16418]  |
| 专题 | 上海光学精密机械研究所_学位论文 |
| 推荐引用方式 GB/T 7714 | 谢承科. 基于ARM 微控制器的上转换发光生物传感器的研制[D]. 中国科学院上海光学精密机械研究所. 2009. |
入库方式： OAI收割
来源：上海光学精密机械研究所
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