基于单片机的上转换磷光生物传感器及其多重检测技术的研究
文献类型：学位论文
| 作者 | 余琨 |
| 学位类别 | 硕士 |
| 答辩日期 | 2007 |
| 授予单位 | 中国科学院上海光学精密机械研究所 |
| 导师 | 黄惠杰 |
| 关键词 | 传感器技术 生物传感器 上转换磷光 免疫分析 |
| 其他题名 | Development of MCU-based UPT Biosensor and Primary Study of the Multi-analyte Detection Technique |
| 中文摘要 | 上转换磷光生物传感器（Up-Converting Phosphor Technology-Based Biosensor, UPT生物传感器）是一种以上转换磷光材料（Up-Converting Phosphor，UCP）为标记物的新型免疫生物传感器。UCP是由稀土元素掺杂于某些晶体晶格中构成的纳米级颗粒，具有在红外光激发下发射出可见磷光的独特性能。在对UCP颗粒进行一系列的表面修饰与活化后，可将其作为生物标记物与多种生物活性分子相结合，应用于生物医学检测领域。运用这种技术可对被检物进行无损伤检测，并且具有检测灵敏度高、速度快、可进行多重检测、使用安全等众多优点。 本论文研制的基于单片机的上转换磷光生物传感器是一台光、机、电一体化的生化检测与分析仪器，由光学系统、数据采集系统、控制系统等几部分组成。在检测过程中，波长为980nm的红外激发光经过激发光路后在试纸条表面形成一个长方形光斑，长方形光斑的长度方向与试纸条的宽度方向一致。试纸条表面的UCP颗粒在红外光激发下发出波长为541.5nm的可见磷光，磷光信号经过磷光接收光路后由PMT转换为电信号。转换后的电信号送入数据采集系统进行分析。通过长方形光斑的扫描检测得到试纸条表面的UCP颗粒分布。单片机系统采集并处理所得到的电信号分布，计算质控带和检测带的位置、检测带信号（T）与质控带信号（C），根据检测带信号与质控带信号的比值（T/C）作出结果判断。 本传感器的特点是：采用单片机系统实现了复杂的控制与数据处理等功能，大大减小了整机体积与功耗，使仪器便于携带，可进行现场检测。 采用一系列浓度（0-1000ng/ml）的标准样品考核了该生物传感器的检测灵敏度、动态响应特性、稳定性等，得到了磷光强度与被检物浓度的响应曲线。检测结果：灵敏度达到ng/ml、以至于亚ng/ml量级，响应曲线的线性相关系数R2≥ 95%，对同一试纸条多次重复检测结果的变异系数CV≤ 5%，满足一般生物监测的需要；多台样机检测结果的一致性优于5%；在内蒙、青海、云南等地现场对上千份实际动物血清及脏器样品进行了检测与分析，仪器性能明显优于常规检测技术。 论文最后介绍了一维多重检测技术的初步实验结果以及多维多重检测生物传感器的技术方案。 |
| 英文摘要 | The UPT-based (Up-Converting Phosphor Technology-Based) Biosensor for UCP (Up-converting phosphor) Immunochromatographic Test Strip is a novel biosensor used in immunoassay, in which UCP is used as the biological marker. As its unique nature, UCP will emit visible phosphorescent light when excited by infrared radiation. After a series of surface modification and activation, it can be used as the marker in biological and medical detection area by combining it with various kinds of biological active molecular. This technique can realize non-invasive detection with merit of rapidness, high sensitivity, stable performance, multi-analyte detection, safe operation, and etc. The MCU-based Biosensor discussed in this dissertation is an optical biosensor which combines optics, mechanics and electronics together. It is made up with several parts, such as optical system, phosphorescent light collector, data processing system, control system and so on. In the process of detection, the test strip is illuminated by a uniform light beam of infrared laser with a wavelength of 980nm, and then the UCP particles will emit some phosphorescent visible light at 541.5nm, which will be imaged onto the sensitive surface of a PMT and transformed into electric signals. The distribution of the voltage value shows the distribution of UCP particles along the test strip. The single chip controller system will then process the signals collected and figure out the relative intensity of T and C. Finally according to the standard calibration curve, the concentration of target analyte can be calculated and displayed on the LCD screen. A characteristic of this biosensor is that it applies a single chip controller to implement the complex controlling and data processing, which reduces the physical dimension and power consumption. It is easy to take and use in the field detection. In order to test the performance of the biosensor, standard samples with serial concentrations varying from 0 to 1000 ng/ml are measured, and satisfied results have been achieved. The results show that the biosensor has higher sensitivity (~ng/ml), high stability (CV≤ 5%), better linear relationship and an excellent correlation (R2≥ 0.95), which is proven the biosensor has stable, reliable and sensitive performances. It can meet the need of various rapid bioassay applications. The practical application results show that the UPT biosensor is more sensitive and quick than the conventional reverse hemagglutination test. Finally, the results of the one-dimensional multiple detection experiments are presented and the primary study of the multi-analyte detection technique is proposed. |
| 语种 | 中文 |
| 源URL | [http://ir.siom.ac.cn/handle/181231/16498]  |
| 专题 | 上海光学精密机械研究所_学位论文 |
| 推荐引用方式 GB/T 7714 | 余琨. 基于单片机的上转换磷光生物传感器及其多重检测技术的研究[D]. 中国科学院上海光学精密机械研究所. 2007. |
入库方式： OAI收割
来源：上海光学精密机械研究所
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