基于上转换发光技术的生物传感器
文献类型：学位论文
| 作者 | 卢健 |
| 学位类别 | 硕士 |
| 答辩日期 | 2005 |
| 授予单位 | 中国科学院上海光学精密机械研究所 |
| 导师 | 黄惠杰 |
| 关键词 | 生物传感器 上转换发光技术 免疫分析 |
| 其他题名 | UPT-based Biosensor |
| 中文摘要 | 生物检测与分析中，在不放大目标物的前提下提高检测的灵敏度和稳定性是检测技术发展的一个重要目标。检测的灵敏度与所采用的标记物和检测仪器有关。许多不同的检测方法在生物医学和临床医学中得到广泛的应用，如：酶、磷光物质、放射性同位素，然而它们都或多或少地存在一些缺陷，从而限制了它们的应用。上转换发光材料（Up－converttngPhosphor，UCP）是由稀土元素掺杂于某些晶体的晶格中构成的纳米级颗粒，具有自然中不存在的反Stokes特性，即在红外光激发下发射可见磷光。UCP拥有一些独特的优点：方便的激发源、可多重分析、无危害性、无背景干扰、无光致漂白、高灵敏度。扫描系统的发展为用UCP标记的分析物的微量检测提供了必要的方法和设备。这些优点与光电检测技术相结合为生物物质的检测提供了可靠的保障。本沦文的主要工作是研究基于上转换发光技术（Up－convertnngPhosphorTechnology，UPT）的生物传感器，该传感器用于检测免疫层析试纸条上经生物反应而结合上去的UCP颗粒的含量，继而计算出被测样品中特定生物分子的浓度。本生物传感器以扫描方式工作，由光学系统、扫描平台、信号采集与处理设备和微机控制系统等几个部分组成。带有被测样品的试纸条被置于扫描平台上，波长为98onm的半导体激光器发出的激光束通过光学系统在试纸条表面聚焦为一条极窄的焦线，焦线处的UCP被激发出磷光（波长范围47－670nm）。产生的磷光被光电转换器转换为电信号，该电信号经信号采集与处理系统采入计算机，经数据处理与分析，得到与被测样品相关的信息。根据仪器的自身特性和应用环境，还开发了针对性的算法和软件，从而较好地弥补了仪器硬件和生物学方面可能带来的影响。论文最后对UPT生物传感器的性能进行了考核。通过对20份系列浓度的兔抗鼠疫IgG标准样品进行检测，结果表明UPT生物传感器的检测灵敏度可达400ng，／ml，其最大变异系数为10％左右，在4O0ng／m卜7000ng／ml浓度范围内具有良好的线性响应特性，这些都表明本传感器具有很好的性能。 |
| 英文摘要 | Improvement of sensitivity and qualification of immunoassay that does not require target-amplification is an important goal in many biological areas. The sensitivity of detection is related to both the type of label employed and the quality and type of equipment available to detect it. Many different types of such detection method are widely used in biomedical research and clinical laboratory medicine. The most common detection labels (reporters) are enzymes, fluorescent dye, and radioisotopes. However, all of them have limitations or problems that narrow their scope and utility. Up-converting Phosphor Technology (UPT) utilizes lanthanide-containing, submicron and ceramic Up-converting phosphor (UCP) particles that possess anti-Stokes shift emission which does not exist in nature. UCP owns several peculiar advantages as follows: convenient excitation source, simultaneous detection of multiple analytes, avoiding potential damaging effect, no background interference, no photobleaching, high sensitivity, and compatible with alternative testing matrices. All of these merits linked with photoelectric instrumentation provide reliable detection in immunoassay. Now the development of scanning system provides method, composition and apparatus for performing minim-detection of analytes by labeling a probe molecule with UCP particles. A UPT-based biosensor for immunochromatography has been developed. The test strips were prepared by conjugation with UCP particles. A scanning system scans the test strip point by point in order to record all the UCP information on it, then figures out the concentration of the biological molecular from the content of the UCP particles on strip. This biosensor is composed of optical system, scanning platform, signal gathering and processing device and microcomputer. The test strip is put on the platform, the laser beam of 980nm is focused on the strip into a line by optical system and the UCP at the line is excited to emit visible light (the range of 475~670nm). Then the light is converted into electric signal by a photomultiplier tube. After the data processed and analyzed, we can get the information of the analyte. As for the software application, some innovative algorithms are developed to make up the shortcomings of hardware and unavoidable factors existing in immunoreaction. To evaluate the performance of the biosensor, analytic samples of serial concentration of have been used. The result indicates that it is effective to carry out precise detection of micro-immunoreaction to the sensitivity of 400ng/ml with fine stability and response in the range from 400ng/ml to 7000ng/ml. |
| 语种 | 中文 |
| 源URL | [http://ir.siom.ac.cn/handle/181231/16503]  |
| 专题 | 上海光学精密机械研究所_学位论文 |
| 推荐引用方式 GB/T 7714 | 卢健. 基于上转换发光技术的生物传感器[D]. 中国科学院上海光学精密机械研究所. 2005. |
入库方式： OAI收割
来源：上海光学精密机械研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。